Efficacy of Sailuotong(塞络通)on neurovascular unit in amyloid precursor protein/presenilin-1 transgenic mice with Alzheimer's disease  

作　　者：SUN Linjuan LI Chengfu LIU Jiangang LI Nannan HAN Fuhua QIAO Dandan TAO Zhuang ZHAN Min CHEN Wenjie ZHANG Xiaohui TONG Chenguang CHEN Dong Qi Jiangxia LIU Yang LIANG Xiao ZHENG Xiaoying ZHANG Yunling 

机构地区：[1]Department of Neurology,Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China [2]China Population and Development Research Center,Beijing 100081,China [3]Department of Institute of Population Research,Peking University,Beijing 100087,China [4]Graduate School of China Academy of Chinese Medical Sciences,Beijing 100700,China [5]Graduate School of Beijing University of Chinese Medicine,Beijing 100029,China

出　　处：《Journal of Traditional Chinese Medicine》2024年第2期289-302,共14页中医杂志（英文版）

基　　金：National Natural Science Foundation of China(81503450):Experimental study on the treatment of transgenic mice with Alzheimer's disease by protecting neurovascular unit by supplementing Qi and activating blood circulation investigate。

摘　　要：OBJECTIVE:To discuss the influence of Sailuotong(塞络通,SLT)on the Neurovascular Unit(NVUs)of amyloid precursor protein(APP)/presenilin-1(PS1)mice and evaluate the role of gas supplementation in activating blood circulation during the progression of Alzheimer's disease(AD).METHODS:The mice were allocated into the following nine groups:(a)the C57 Black(C57BL)sham-operated group(control group),(b)ischaemic treatment in C57BL mice(the C57 ischaemic group),(c)the APP/PS1 sham surgery group(APP/PS1 model group),(d)ischaemic treatment in APP/PS1 mice(APP/PS1 ischaemic group),(e)C57BL mice treated with aspirin following ischaemic treatment(C57BL ischaemic+aspirin group),(f)C57BL mice treated with SLT following ischaemic treatment(C57BL ischaemic+SLT group),(g)APP/PS1 mice treated with SLT(APP/PS1+SLT group),(h)APP/PS1 mice treated with donepezil hydrochloride following ischaemic treatment(APP/PS1 ischaemic+donepezil hydrochloride group)and(i)APP/PS1 mice treated with SLT following ischaemic treatment(APP/PS1 ischaemic+SLT group).The ischaemic model was established by operating on the bilateral common carotid arteries and creating a microembolism.The Morris water maze and step-down tests were used to detect the spatial behaviour and memory ability of mice.The hippocampus of each mouse was observed by haematoxylin and eosin(HE)and Congo red staining.The ultrastructure of NVUs in each group was observed by electron microscopy,and various biochemical indicators were detected by enzymelinked immunosorbent assay(ELISA).The protein expression level was detected by Western blot.The mRNA expression was detected by quantitative real-time polymerase chain reaction(qRT-PCR).RESULTS:The results of the Morris water maze and step-down tests showed that ischemia reduced learning and memory in the mice,which were restored by SLT.The results of HE staining showed that SLT restored the pathological changes of the NVUs.The Congo red staining results revealed that SLT also improved the scattered orange-red sediments in the upper cortex a

关 键 词：Alzheimer disease amyloid beta-protein precursor PRESENILIN-1 mice TRANSGENIC replenishing Qi and activating blood neurovascular unit Sailuotong 

分 类 号：R285.5[医药卫生—中药学]