UHPLC-MS/MS法测定小鼠血浆和脑中的4F-MDMB-BUTICA含量  

Determination of 4F-MDMB-BUTICA in Mice Plasma and Brain by UHPLC-MS/MS

作　　者：许雅雯 李香豫 徐鹏 乔艳玲 严方[1,2] 王丹[2,3] XU Yawen;LI Xiangyu;XU Pengg;QIAO Yanlingg;YAN Fang;WANG Dan(School of Pharmacy,China Pharmaceutical University,Nanjing 210009,Jiangsu,China;Ofice of China National Narcotics Control Commission,China Pharmaceutical University Joint Laboratory on Key Technologies of Narcotics Control,Beijing 100193,China;Key Laboratory of Drug Monitoring and Control,Drug Intelligence and Forensic Center,Ministry of Public Security,Beijing 100193,China)

机构地区：[1]中国药科大学,江苏南京210009 [2]国家禁毒委员会办公室-中国药科大学禁毒关键技术联合实验室,北京100193 [3]毒品监测管控与禁毒关键技术公安部重点实验室,公安部禁毒情报技术中心,北京100193

出　　处：《中国药物滥用防治杂志》2024年第4期587-591,608,共6页Chinese Journal of Drug Abuse Prevention and Treatment

基　　金：公安部科技强警基础工作专项《吸入型物质成瘾性和危害性评估技术》(编号:2022JC19);国家重点研发计划(编号:2022YFC3300905)。

摘　　要：目的:建立液相色谱-串联质谱法(UHPLC-MS/MS)测定小鼠血液和脑组织样本中4F-MDMB-BUTICA的含量。方法:采用液相色谱串联质谱法,使用C_(18)色谱柱,以0.1%甲酸水和乙腈为流动相,以5F-MDMB-PICA为内标物质,通过液-液萃取前处理后对滥用4F-MDMB-BUTICA后小鼠的血液和脑组织样本中的4F-MDMB-BUTICA进行定量检测,并对该方法进行方法学验证。结果:血浆和脑组织中的4F-MDMB-BUTICA在0.1~100 ng/ml的范围内均线性关系良好(R^(2)分别为0.9942和0.9939),最低检出限为0.1 ng/ml。日内准确度和日间准确度均在85%~115%范围内,日内精密度和日间精密度均小于15%,且基质效应的影响在85%~115%范围内,表明该方法下基质无明显增强或抑制效应。结论:本研究建立了一种准确、灵敏、可靠的检测方法使用液相色谱串联质谱法(UHPLC-MS/MS)检测血液和脑组织样本中4F-MDMBBUTICA,为该物质基于动物的相关成瘾研究做基础。Objective:To establish a liquid chromatography tandem mass spectrometry(UHPLC-MS/MS)method for the determination of 4F-MDMB-BUTICA in mice blood and brain tissues.Methods:Liquid chromatography tandem mass spectrometry was used,with a C_(18)reverse phase chromatographic column and 0.1%formic acid water and acetonitrile as mobile phases.The liquid-liquid extraction was selected as pre-treatment method,and 5F-MDMB-PICA was used as the internal standard substance to quantitatively detect 4F-MDMB-BUTICA in blood and brain tissue samples of mice after an abuse of 4F-MDMB-BUTICA.The methodology was validated.Results:There was a good linear relationship between 4F-MDMB-BUTICA in plasma and brain tissue in the range of 0.1-100 ng/ml(R^(2)was 0.9942 and 0.9939,respectively),with a minimum detection limit of 0.1 ng/ml.The intra day accuracy and inter day accuracy were both between 85%and 115%,and intra day precision and inter day precision were less than 15%.The matrix efect was in the range from 85%to 115%,indicating that there was no signifcant enhancement or inhibition efect in this method.Conclusion:This study established an accurate,sensitive,and reliable detection method for 4F-MDMB-BUTICA in blood and brain tissue samples using liquid chromatography tandem mass spectrometry(UHPLC-MS/MS),laying the foundation for animal related addiction research on this substance.

关 键 词：液质联用法 4F-MDMB-BUTICA 含量测定 合成大麻素类物质 

分 类 号：R99[医药卫生—毒理学]