MMP25和E-cadherin在类风湿关节炎中的诊断价值  

Diagnostic value of MMP25 and E-cadherin in rheumatoid arthritis

作　　者：方全钢[1] 刘海芸[1] 万静[2] 张水生[1] FANG Quangang;LIU Haiyun;WAN Jing;ZHANG Shuisheng(Department of Clinical Laboratory,Jiangxi Provincial People's Hospital(The First Affiliated Hospital of Nanchang Medical College),Jiangxi,Nanchang 330006;Department of Ophthalmology,Jiangxi Provincial People's Hospital(The First Affiliated Hospital of Nanchang Medical College),Jiangxi,Nanchang 330006)

机构地区：[1]江西省人民医院南昌医学院第一附属医院检验科,南昌330006 [2]江西省人民医院南昌医学院第一附属医院眼科,南昌330006

出　　处：《江西医药》2024年第2期143-147,154,共6页Jiangxi Medical Journal

基　　金：江西省卫生健康委科技计划课题,编号202130043。

摘　　要：目的探讨基质金属蛋白酶25(Matrix metalloproteinase 25,MMP25)和E-钙黏蛋白(E-cadherin)对类风湿关节炎的诊断意义。方法选取2020年1月至2023年10月我院67例类风湿关节炎患者为实验组,同期50例健康体检人群为对照组;采用酶联免疫吸附试验(ELISA)检测二组血清中MMP25和E-cadherin蛋白水平。收集实验组患者ESR、CRP、CCP、RF、DAS28-ESR、DAS28-CRP指标,以及是否为初诊、是否合并并发症(贫血、间质性肺炎等)、病程、临床活动度等级,并与MMP25和E-cadherin水平进行相关性分析。结果实验组血清MMP25和E-cadherin水平均高于对照组,差异均有统计学意义(P<0.001)。与ESR、CRP、CCP和RF等指标进行散点图和简单线性回归性分析,结果显示:不同临床活动度患者表达MMP25水平差异有统计学意义(P<0.001),MMP25与病程(β=0.479,P<0.001),MMP25与CRP(β=0.271,P<0.05),MMP25与DAS28-ESR(β=0.660,P<0.001)和MMP25与DAS28-CRP(β=0.624,P<0.001)均有相关性。不同临床活动度患者表达E-cadherin水平差异有统计学意义(P<0.001),E-cadherin与ESR(β=0.425,P<0.001),E-cadherin与CRP(β=0.328,P<0.05),E-cadherin与DAS28-ESR(β=0.746,P<0.001),E-cadherin与DAS28-CRP(β=0.671,P<0.001)均有相关性。多重线性回归分析结果显示,MMP25表达水平与临床活动度正相关(β=1.032,P<0.001),E-cadherin表达水平与临床活动度正相关(β=0.407,P<0.05)、与病程长短正相关(β=0.497,P<0.001)。结论MMP25和E-cadherin表达水平可以作为类风湿关节炎评估临床活动度的标志物,此外,E-cadherin表达水平也可以衡量患者病程长短。Objective To investigate the diagnostic significance of Matrix metalloproteinase 25(MMP25)and E-cadherin in rheumatoid arthritis.Methods 67 patients with rheumatoid arthritis in our hospital from January 2020 to October 2023 were chosen as the experimental group,and 50 healthy people during the same period were chosen as the control group.The serum levels of MMP25 and E-cadherin were detected by enzyme-linked immunosorbent assay(ELISA).The indexes of ESR,CRP,CCP,RF,DAS28-ESR and DAS28-CRP were collected from patients in the experimental group,as well as whether they were newly diagnosed,whether they had complications(anemia,interstitial pneumonia,etc),disease course and clinical activity grade and correlation with MMP25 and E-cadherin levels were analyzed.Results The serum levels of MMP25 and E-cadherin in rheumatoid arthritis patients were higher than those in control group,with statistical significance(P<0.001).Scatter plot and simple linear regression analysis with ESR,CRP,CCP and RF showed that there were significant differences in the expression of MMP25 in different clinical activity groups(P<0.001),MMP25 and disease course(β=0.479,P<0.001),MMP25 and CRP(β=0.271,P<0.05),MMP25 and DAS28-ESR(β=0.660,P<0.001)and MMP25 and DAS28-CRP(β=0.624,P<0.001)are all correlated;There were significant differences there were significant differences in the expression of E-cadherin in different clinical activity groups(P<0.001),E-cadherin and ESR(β=0.425,P<0.001),E-cadherin and CRP(β=0.328,P<0.05),E-cadherin and DAS28-ESR(β=0.746,P<0.001),E-cadherin and DAS28-CRP(β=0.671,P<0.001).The results of multiple linear regression analysis showed that the expression level of MMP25 was positively correlated with clinical activity(β=1.032,P<0.001),the expression level of E-cadherin was positively correlated with clinical activity(β=0.407,P<0.05),and the duration of disease(β=0.497,P<0.001).Conclusion The expression level of MMP25 and E-cadherin can be used as markers to evaluate clinical activity in rheumatoid arthritis,and th

关 键 词：基质金属蛋白酶25 E-钙黏蛋白 类风湿关节炎 诊断价值 

分 类 号：R593.22[医药卫生—内科学]