“心痛三两三”方对异丙肾上腺素引起的斑马鱼心肌缺血的作用  

The Effect of“XinTong-SanLiangSan”Formula on Myocardial Ischemia Induced by Isoproterenol in Zebrafish

作　　者：陈思琪 郭帅杰 解长旭 张蕾[1,2] 刘卫红 李思耐[1,2] 刘红旭 周明学[1,2] CHEN Siqi;GUO Shuaijie;XIE Changxu;ZHANG Lei;LIU Weihong;LI Sinai;LIU Hongxu;ZHOU Mingxue(Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010,China;Beijing Institute of Traditional Chinese Medicine,Beijing 100010,China)

机构地区：[1]首都医科大学附属北京中医医院,北京100010 [2]北京市中医药研究所,北京100010

出　　处：《世界中医药》2024年第6期802-806,共5页World Chinese Medicine

基　　金：国家自然科学基金项目(82274287);北京市自然科学基金项目(7232266)。

摘　　要：目的:研究“心痛三两三”方对异丙肾上腺素引起的斑马鱼心肌缺血的作用。方法:用“心痛三两三”方9.4、18.75、37.5、75、150和300μL/mL浓度干预受精后2~3 d野生型AB品系斑马鱼,根据斑马鱼的毒性表型和死亡情况确定“心痛三两三”方的最大耐受浓度,从而筛选出本研究的给药剂量。实验设“心痛三两三”方高、中、低剂量组,浓度分别为75.0、25,0、8.3μL/mL,阳性对照丹参滴丸组浓度为500μg/mL,正常对照组和模型对照组,每组30尾置于六孔板中,各组均置于28℃培养箱中培养4 h后,用50 mg/mL盐酸异丙肾上腺素诱导60 min建立斑马鱼心肌缺血模型。共同处理结束后,用吖啶橙法染色分析斑马鱼心脏部位凋亡细胞荧光强度;心跳血流分析软件录制斑马鱼血流视频,分析斑马鱼心输出量和血流速度。结果:与模型对照组比较,“心痛三两三”方中、高剂量组斑马鱼心脏部位凋亡细胞荧光强度显著降低(P<0.05,P<0.01),其凋亡改善率分别为34%和54%。“心痛三两三”方低、中、高剂量组斑马鱼心输出量和血流速度明显提高(P<0.01),其改善率分别为52%、93%、62%和29%、53%、32%。结论:“心痛三两三”方对异丙肾上腺素诱导的斑马鱼心肌缺血具有明显改善作用,与改善心肌细胞凋亡有关。Objective:To study the preventive and therapeutic effect of“XinTong-SanLiangSan”Formula on myocardial ischemia induced by isoproterenol in zebrafish.Methods:The concentrations of 9.4,18.75,37.5,75,150 and 300μL/mL of the“XinTong-SanLiangSan”Formula were used to intervene wild-type AB strain zebrafish 2 to 3 days after fertilization.The maximum tolerance concentration of the“XinTong-SanLiangSan”Formula was determined according to the toxicity phenotype and mortality of zebrafish,thereby screening out the dosage for this study.In this experiment,the high,medium and low dose groups of“XinTong-SanLiangSan”Formula were set up,the concentrations were 75,25 and 8.3μL/mL,respectively,the concentration of the positive control Danshen dripping pills group was 500μg/mL,the normal control group and the model control group.30 fish in each group were placed in a six-well plate,each group was placed in an incubator at 28℃for 4 h,and then induced with isoproterenol hydrochloride(50 mg/mL)for 60 min to establish the myocardial ischemia model of zebrafish.After the treatment,the fluorescence intensity of apoptotic cells in the heart of zebrafish was analyzed by acridine orange staining.The zebrafish blood flow video was recorded by the heartbeat blood flow analysis software to analyze the cardiac output and blood flow velocity of zebrafish.Results:Compared with the model control group,the fluorescence intensities of apoptotic cells in the heart of zebrafish in the medium and high dose groups of“XinTong-SanLiangSan”Formula group were significantly decreased(P<0.05,P<0.01),and the improvement rates of apoptosis were 34%and 54%,respectively.The cardiac output and blood flow velocity of zebrafish in low,medium and high dose groups of“XinTong-SanLiangSan”Formula group were significantly increased(P<0.01),and the improvement rates were 52%,93%,62%and 29%,53%,32%,respectively.Conclusion:“XinTong-SanLiangSan”Formula can significantly improve myocardial ischemia induced by isoproterenol in zebrafish,which i

关 键 词：“心痛三两三”方 斑马鱼 心肌缺血 细胞凋亡 盐酸异丙肾上腺素 胸痹 心输出量 血流速度 

分 类 号：R285.5[医药卫生—中药学] R-322[医药卫生—中医学]