转基因T_(1)代番茄对黄化曲叶病抗性分析  

Resistance Analysis of Transgenic T_(1)Tomato to Yellow Leaf Curl Disease

作　　者：姜睿鹏 朱晓林 王宝强[1] 魏小红[1] JIANG Ruipeng;ZHU Xiaolin;WANG Baoqiang;WEI Xiaohong(College of Life Science and Technology,Gansu Agricultural University,Lanzhou,Gansu 730700;College of Agronomy,Gansu Agricultural University,Lanzhou,Gansu 730700)

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730700 [2]甘肃农业大学农学院,甘肃兰州730700

出　　处：《北方园艺》2024年第9期1-10,共10页Northern Horticulture

基　　金：国家自然科学基金资助项目(32060401);甘肃省自然科学基金资助项目(21JR7RA808)。

摘　　要：以转黄化曲叶病(TYLCV)编码外壳蛋白(capsid protein,CP)的V1、编码复制激发蛋白(replication-associated protein,Rep)C1基因的T_(1)代J6番茄为试材,采用盆栽法,研究转基因T_(1)代番茄接种TYLCV后不同时期的生长特性、抗病相关酶活性和抗病相关基因表达量的影响,以期明确转V1、C1基因的T_(1)代J6番茄植株对TYLCV的抗性。结果表明:通过RNAi技术获得的转V1、C1基因的T_(1)代转基因J6番茄植株成功转化且供试番茄材料转化率为87.5%;T_(1)代转基因植株抗病性研究发现:转基因番茄可以有效缓解病害对其生长的抑制,光合特性得到一定程度的恢复;其抗病相关酶PAL、PPO活性下降;MDA和抗氧化相关酶超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)和过氧化物酶(POD)活性显著降低;抗病相关基因的表达量Ty-1、Ty-2、Ty-3显著上调。说明T_(1)代转基因番茄对TYLCV具有一定的抗性,且J6+V1的抗病性强于J6+C1。In order to clarify the resistance of T_(1)generation J6 tomato plants transformed with V1 and C1 genes to TYLCV,the V1 gene encoding capsid protein(CP)of TYLCV and the J6 gene encoding replication-associated protein(Rep)C1 were used as test materials to study the effects of TYLCV on the growth characteristics,disease resistance-related enzyme activities and disease resistance-related gene expression at different stages after inoculation with TYLCV by pot experiment.The results showed that the T_(1)transgenic J6 tomato plants with V1 and C1 genes obtained by RNAi technology were successfully transformed and the transformation rate of the tested tomato materials was 87.5%.The study of disease resistance of T_(1)generation transgenic plants found that transgenic tomatoes could limitedly alleviate the inhibition of disease on their growth,and the photosynthetic characteristics were restored to a certain extent.The activities of PAL and PPO related to disease resistance decreased.The MDA content and activities of antioxidant enzymes superoxide dismutase(SOD),catalase(CAT),ascorbate peroxidase(APX)and peroxidase(POD)were significantly decreased.The expression levels of resistance-related genes Ty-1,Ty-2 and Ty-3 were significantly up-regulated.The results showed that the T_(1)generation of transgenic tomatoes had certain resistance to TYLCV,and the resistance of‘J6+V1’was stronger than that of‘J6+C1’.

关 键 词：转基因番茄 T_(1) TYLCV 抗病性 

分 类 号：S641.2[农业科学—蔬菜学]