等离子体活化水对根癌土壤杆菌的抑制作用及其对樱花根癌病的防治  

Inhibitory Effect of Plasma Activated Water on Agrobacterium tumefaciens and Its Prevention and Treatment of Cerasus Crown Gall

作　　者：潘越 司红波[2] 潘宇伟 孙永莲 PAN Yue;SI Hongbo;PAN Yuwei;SUN Yonglian(Laboratory and Base Construction Management Department,Nanjing Forestry University,Nanjing,Jiangsu 210037;Logistics Management Division,Nanjing Forestry University,Nanjing,Jiangsu 210037)

机构地区：[1]南京林业大学实验室与基地建设管理处,江苏南京210037 [2]南京林业大学后勤管理处,江苏南京210037

出　　处：《北方园艺》2024年第9期54-61,共8页Northern Horticulture

基　　金：国家重点研发计划资助项目(2019YFD1002300);江苏高校哲学社会科学研究资助项目(2023SJYB0161)。

摘　　要：以樱花幼苗为试材,使用低温等离子体活化水冷杀菌技术(PAW)对樱花幼苗及其病原菌进行处理,研究了PAW对樱花苗木的抗病作用,以期为PAW在樱花根癌病防治中的应用提供参考依据。结果表明:等离子体活化水能有效地抑制樱花根癌病病菌Agrobacterium tumefaciens的生长,其可以对Agrobacterium tumefaciens的细胞结构造成破坏。CK组樱花幼苗的根瘤发生率为100%,根瘤的平均直径为18.8 mm,处理后的幼苗发病率为56.7%,根瘤的平均直径为8.4 mm。此外,PAW可以提高樱花根系多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)及超氧化物歧化酶(SOD)活性的表达,激活樱花植株的抗性。综上,PAW具有一定的生物防治潜力,可以防御樱花根癌病。Taking cherry blossom seedlings as the test material,PAW was used to treat cherry blossom seedlings and their pathogens,and the anti disease effect of PAW on cherry blossom seedlings was studied,in order to provide reference for the application of PAW in the prevention and control of Cerasus crown gall.The results showed that plasma activated water can effectively inhibit the growth of cherry blossom root cancer pathogen Agrobacterium tumefaciens,which could cause damage to the cellular structure of Agrobacterium tumefaciens.The incidence of root nodules of cherry seedlings in the control group was 100%,the average diameter of root nodules was 18.8 mm,the incidence rate of treated seedlings was 56.7%,and the average diameter of root nodules was 8.4 mm.In addition,PAW could increase the expression of PPO,PAL and SOD activities in cherry blossom roots,activating the resistance of cherry blossom plants.In summary,PAW had certain biological control potential and could defend against Cerasus crown gall.

关 键 词：樱花 根癌病 Agrobacterium tumefaciens 等离子体活化水 

分 类 号：S474[农业科学—农业昆虫与害虫防治]