基因重配对B型流感病毒生长特性的影响  

Effect of gene reassortment on growth characteristics of influenza B virus

作　　者：张瑶 周剑芳[2] 鲁健[2] 李希妍[2] 曾晓旭[2] 王大燕[2] ZHANG Yao;ZHOU Jianfang;LU Jian;LI Xiyan;ZENG Xiaoxu;WANG Dayan(Microbiological Laboratory,Dongcheng District Center for Disease;不详)

机构地区：[1]北京市东城区疾病预防控制中心检验科,北京100050 [2]中国疾病预防控制中心,病毒病预防控制所世界卫生组织流感参比和研究合作中心,北京102206

出　　处：《中国生物制品学杂志》2024年第4期426-432,共7页Chinese Journal of Biologicals

基　　金：国家科技重大专项(2020ZX10001016)。

摘　　要：目的 利用反向遗传学方法研究流感病毒各基因节段对其生长特性的影响。方法 挑选系间重配病毒B/福建同安/1565/2013及B/北京怀柔/11764/2013进行生长曲线测定。提取病毒核酸,扩增病毒各基因节段,并利用pHW2000载体构建重组质粒。对构建的重组质粒在两病毒间进行单一及不同组合基因节段的替换后,构建新的重配病毒,检测重配病毒的生长特性,推测基因节段的影响作用。结果 2株系间重配病毒生长特性差异显著,B/福建同安/1565/2013比B/北京怀柔/11764/2013生长曲线的峰值点高约2倍log值,但将B/北京怀柔/11764/2013单一节段分别替换至B/福建同安/1565/2013重配出的8株病毒与B/福建同安/1565/2013的生长特性差异不明显,而在同时替换HA和NA(表面蛋白)或PB2、PB1和PA(聚合酶)或PB2、PB1、PA和NP(核糖核蛋白,RNP)3种基因组合时,重配病毒的生长特性下降显著。结论 B型流感病毒生长特性受单一节段影响可能性较小,多个节段的协同作用对其影响更为显著,在监测过程中应加强对重配变异病毒的监测。Objective To study the influence of various gene segments of influenza virus on its growth characteristics by using the reverse genetics system.Methods The growth curves of inter⁃lineage reassortment viruses B/FujianTongan/1565/2013 and B/BeijingHuairou/11764/2013 were determined.The nucleic acids of the two strains were extracted and all gene segments were amplified,then pHW2000 vector was used to construct recombinant plasmids of all segments.The reassortment B/FujianTongan/1565/2013 virus was constructed,then single or different combinations of segments were replaced by segments from B/BeijingHuairou/11764/2013 virus to construct new reassortment viruses.The growth characteristics of all reassortment viruses were analyzed to identify the influence of the gene segments.Results There was significant difference in growth characteristics between the two strains,and the peak value of the growth curve of B/FujianTongan/1565/2013 was about 2 times log higher than that of B/BeijingHuairou/11764/2013.However,there was no significant difference in growth characteristics between B/FujianTongan/1565/2013 and the 8 strains of reassortment viruses with single segment replaced by segment from B/BeijingHuairou/11764/2013,but when the gene combination of HA and NA(surface protein)or PB2,PB1 and PA(polymerase)or PB2,PB1,PA and NP(ribonucleo⁃protein,RNP)were replaced,the growth characteristics of the reassortment virus decreased significantly.Conclusion The growth characteristics of influenza B virus may be less affected by a single segment,and the synergistic effect of multiple segments is more significant.Therefore,the monitoring of reassortment variant viruses should be strengthened in the surveillance process.

关 键 词：B型流感病毒 基因重配 反向遗传 生长特性 

分 类 号：Q93-33[生物学—微生物学]