辐毛小鬼伞对杜鹃兰种子萌发的促进作用  

Study on the Promoting Effect of Coprinellus radians on Seed Germination of Cremastra appendiculata

作　　者：王静[1] 潘长能 王淙薇 何姗姗 田爱华 刘文凯 李小军[1] WANG Jing;PAN Zhang-neng;WANG Cong-wei;HE Shan-shan;TIAN Ai-hua;LIU Wen-kai;LI Xiao-jun(College of Pharmacy,South-Central Minzu University,Wuhan 430074,China;Hubei Province Badong 108 Medicinal Planting Professional Cooperative,Badong 444300,China;Hubei Hefeng County Linyuan Forestry Development Co.,Ltd.,Hefeng 445800,China)

机构地区：[1]中南民族大学药学院,湖北武汉430074 [2]湖北省巴东一零八药材种植专业合作社,湖北巴东444300 [3]湖北鹤峰县林源林业发展有限公司,湖北鹤峰445800

出　　处：《中药材》2023年第11期2658-2662,共5页Journal of Chinese Medicinal Materials

基　　金：中南民族大学中央高校基本科研业务费专项(CXY21013,CZY20046,CXY21005)。

摘　　要：目的:研究辐毛小鬼伞对杜鹃兰种子萌发的促进作用,提供一种简便培育杜鹃兰幼苗的方法。方法:收集铺满棕色“地毯状”菌丝且长有能变黑自溶蘑菇(疑似辐毛小鬼伞)的腐木,将其切段后放入自封袋中,撒上杜鹃兰种子进行共生培养,待种子萌发形成原球茎后,分离内生菌,并用内生菌接种菌包培育其子实体,进行形态学观察及ITS序列鉴定,再进行促进杜鹃兰种子萌发试验。结果:此菌株能促进杜鹃兰种子萌发,培育出的子实体特征与原收集的菌株一致。根据形态学特征及ITS序列鉴定此菌株为辐毛小鬼伞Coprinellus radians。结论:辐毛小鬼伞能促进杜鹃兰种子萌发,为促进杜鹃兰繁育提供了有效的方法。Objective:To study the promoting effect of Coprinellus radians on seed germination of Cremastra appendiculata,so as to provide a simple method for cultivating Cremastra appendiculata seedlings.Methods:The decayed wood covered with brown"carpeted"mycelia and containing blackened autolytic mushrooms(suspected to be Coprinellus radians)was collected and cut into segments,placed into a self-sealing bag.Cremastra appendiculata seeds were scattered on the segments for symbiotic culture.After the seeds germinated to form protocorms,the endophytes were isolated and inoculated into the bag to cultivate the fruiting body.Morphological observation and ITS sequence identification were performed.The experiment of promoting seed germination of Cremastra appendiculata was carried out.Results:The strain was found to promote seed germination of Cremastra appendiculata,and the fruiting body characteristics of the cultivated strain were consistent with those of the collected strain.According to morphological feature and ITS sequence,the strain was identified as Coprinellus radians.Conclusion:Coprinellus radians can promote seed germination of Cremastra appendiculata,which provides effective method for its breeding and development.

关 键 词：辐毛小鬼伞 杜鹃兰 共生培养 种子萌发 

分 类 号：R282.2[医药卫生—中药学]