桑叶水提物对2型糖尿病小鼠皮下脂肪组织氧化应激的影响及机制  

Effect and Mechanism of Water Extract of Mori Folium on Oxidative Stress in Adipose Tissue of T2DM Mice

作　　者：张慧琳 安永铖 何昶昊 黄艳 付宛鑫 王梦璐 单子轶 杜雨航 解佳美 贾占红[1] 赵保胜 ZHANG Huilin;AN Yongcheng;HE Changhao;HUANG Yan;FU Wanxin;WANG Menglu;SHAN Ziyi;DU Yuhang;XIE Jiamei;JIA Zhanhong;ZHAO Baosheng(College of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;College of Life Sciences,Beijing University ofChinese Medicine,Beijing 102488,China;Beijing Research Institute of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学中药学院,北京102488 [2]北京中医药大学生命科学学院,北京102488 [3]北京中医药大学北京中医药研究院,北京100029

出　　处：《中国实验方剂学杂志》2024年第11期43-50,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(8227142742,8197140715)。

摘　　要：目的:观察桑叶水提物(MLE)对2型糖尿病(T2DM)小鼠脂肪组织氧化应激的影响,并探讨其作用机制。方法:24只雄性db/db小鼠按血糖、体质量随机分为模型组、二甲双胍组、桑叶水提物低剂量(MLE-L)组和桑叶水提物高剂量(MLE-H)组,每组6只;另设6只C57BLKS/JGpt同窝野生型(m/m)小鼠为正常组。二甲双胍组给予200 mg·kg^(-1)二甲双胍溶液,MLE-L组、MLE-H组分别给予2、4 g·kg^(-1)MLE,正常组和模型组均给予等体积去离子水,每日灌胃1次,连续给药8周。测定小鼠体质量、皮下脂肪指数、空腹血糖(FBG)及口服糖耐量水平,检测血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)含量,蛋白免疫印迹法(Western blot)检测小鼠皮下脂肪组织中沉默信息调节因子1(SIRT1)、NADPH氧化酶4(NOX4)蛋白的表达水平。结果:与空白组比较,T2DM小鼠经桑叶水提物给药后,空腹血糖水平、口服糖耐量水平(OGTT)、脂肪组织指数均明显下降(P<0.05,P<0.01);血清抗氧化物酶SOD、GSH含量明显上升,氧化应激损伤标志物MDA水平明显下降(P<0.05,P<0.01);脂肪组织中SIRT1蛋白表达显著上升、NOX4蛋白表达明显下降(P<0.05,P<0.01)。结论:桑叶水提物可通过缓解T2DM小鼠脂肪组织氧化应激状态,从而降低血糖,改善糖尿病的作用。Objective: To observe the effect of water extract of Mori Folium(MLE) on oxidative stress in adipose tissue of type 2 diabetes mellitus(T2DM) mice and explore its mechanism. Method: Twenty-four male db/db mice were randomly divided into model group, metformin group, low-dose MLE(MLE-L) group, and high-dose MLE(MLE-H) group according to their body weight and blood glucose, with six mice in each group, and other six C57BLKS/JGpt wild littermate mice were selected as normal group. The mice in the metformin group were given 200 mg·kg^(-1) metformin suspension, and the mice in the MLE-L and MLE-H groups were respectively given 2 g·kg^(-1) and 4 g·kg^(-1) MLE, while the mice in the normal group and model group were given the same dose of deionized water by daily gavage for eight weeks. Body weight, subcutaneous fat index, fasting blood glucose(FBG), and oral glucose tolerance level(OGTT) of the mice were detected, and serum superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), and malondialdehyde(MDA) were measured. The expression levels of silent information regulator 1(SIRT1) and NADPH oxidase type 4(NOX4) protein in subcutaneous adipose tissue of the mice were detected by Western blot. Result: The FBG level, OGTT, and subcutaneous fat index of T2DM mice were significantly decreased(P<0.05, P<0.01) after administration of MLE compared with the blank group. The contents of serum SOD and GSH were significantly increased, while the level of oxidative stress damage marker MDA was significantly decreased(P<0.05, P<0.01). The expression of SIRT1 protein in adipose tissue was significantly increased, while the expression of NOX4 protein was significantly decreased(P<0.05, P<0.01). Conclusion: MLE can ameliorate T2DM by alleviating oxidative stress in adipose tissue of T2DM mice and reducing blood glucose.

关 键 词：桑叶水提物 2型糖尿病 氧化应激 脂肪组织 

分 类 号：R2-0[医药卫生—中医学] R22R242R285.5R856.2