酸枣选育新品种遗传多样性的SCoT标记分析  

SCoT Lakel Analysis on Genetic Diversity of New Cultivars of Ziziphus jujuba var.spinosa

作　　者：王琳[1] 赵红艳 王云达 杜宪臣 曹岩 郭庆梅[1] WANG Lin;ZHAO Hong-yan;WANG Yun-da;DU Xian-chen;CAO Yan;GUO Qing-mei(Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Jinan Zijinmeigui Co.,Ltd.,Jinan 250407,China;Pingyin County Natural Resources Bureau,Jinan 250499,China)

机构地区：[1]山东中医药大学,山东济南250355 [2]济南紫金玫瑰股份有限公司,山东济南250407 [3]平阴县自然资源局,山东济南250499

出　　处：《中药材》2023年第10期2425-2429,共5页Journal of Chinese Medicinal Materials

基　　金：山东省自然科学基金项目(ZR2021MH123);济南市农业应用技术创新计划项目(CX202110);山东省科技型中小企业创新能力提升工程项目(2022TSGC2181)。

摘　　要：目的:利用SCoT分子标记技术分析酸枣选育新品种及酸枣栽培品种的遗传多样性和亲缘关系,为酸枣种质资源的保护、品种改良和优化夯实研究基础。方法:从100个SCoT引物中筛选出条带重复性好、多态性高的引物对供试材料进行PCR扩增,计算不同酸枣品种间的遗传相似系数,用非加权组平均距离法(UPGMA)进行聚类分析。结果:从100个SCoT引物中筛选出17个引物,共扩增出210条片段,每个引物平均产生12.35条片段。多态性条带187条,多态性比率为89.05%。22个酸枣品种间的遗传相似系数为0.561~0.941。聚类分析结果显示,在遗传相似系数为0.72处22个酸枣品种聚为三组。结论:该研究对酸枣种质资源的鉴定保护,分子辅助育种和开发利用提供了理论依据。Objective:To analyze the genetic diversity and genetic relationship of new varieties and cultivated varieties of Ziziphus jujuba var.spinosa germplasm by SCoT molecular marker technology,so as to lay a solid research foundation for the protection,improvement and optimization of the germdasm resources of Ziziphus jujuba var.spinosa.Methods:The primers with good band repeatability and high polymorphism were screened from 100 SCoT primers for PCR amplification of the test materials,and the genetic similarity coefficient between varieties was calculated.The cluster analysis was performed by unweighted average distance method(UPGMA).Results:17 primers were screened out from 100 SCoT primers,a total of 210 fragments were amplified,and each primer produced an average of 12.35 fragments.There were 187 polymorphic bands,and the polymorphism ratio was 89.05%.The genetic similarity coefficients of 22 Ziziphus jujuba var.spinosa germplasms were 0.561 to 0.941.The cluster analysis results showed that at a genetic similarity coefficient of 0.72,22 germplasms were clustered into three groups.Conclusion:This study provides a theoretical basis for identification and protection of germplasm resources,molecular assisted breeding and exploitation of Ziziphus jujuba var.spinosa germplasm resources.

关 键 词：酸枣 新品种 亲缘关系 遗传多样性 SCoT分子标记 

分 类 号：R282.71[医药卫生—中药学]