民族药四数九里香的化学成分及其细胞毒活性研究(Ⅳ)  

Chemical Constituents of Ethnodrug Murraya tetramera and the Cytotoxic Activity(Ⅳ)

作　　者：周永福 姚如杰[1] 杨成威 黎学明 ZHOU Yong-fu;YAO Ru-jie;YANG Cheng-wei;LI Xue-ming(Technology Center of Chongqing Zi-Guang Chemical Co.,Ltd.,Chongqing 401121,China;College of Chemistry and Pharmaceutical Engineering,Chongqing Industry Polytechnic College,Chongqing 401120,China;College of Chemistry and Chemical Engineering,Chongqing University,Chongqing 400044,China)

机构地区：[1]重庆紫光化工股份有限公司技术中心,重庆401121 [2]重庆工业职业技术学院化学与制药工程学院,重庆401120 [3]重庆大学化学化工学院,重庆400044

出　　处：《中药材》2023年第10期2475-2480,共6页Journal of Chinese Medicinal Materials

基　　金：重庆紫光化工股份有限公司博士后项目(KQY20220530)。

摘　　要：目的:研究民族药四数九里香Murraya tetramera Huang的化学成分。方法:采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱等技术分离纯化化合物,采用波谱方法鉴定化合物结构;采用MTS法分析单体化合物的细胞毒活性。结果:从四数九里香醇提物的乙酸乙酯萃取部位中分离得到15个化合物,分别鉴定为:苜蓿素-7-O-β-D-葡萄糖苷(1)、6-甲氧基香豆素-7-O-β-D-吡喃葡萄糖苷(2)、N-乙酰谷氨酸(3)、鼠尾草素(4)、去氢骆驼蓬碱(5)、piperumbellactam A(6)、salutaridine(7)、murradiol(8)、(Z)-2,3,5,4′-四羟基-二苯乙烯-2-O-β-D-葡萄糖苷(9)、芹菜素(10)、piperdardine(11)、紫云英苷(12)、β-香树脂醇(13)、原儿茶酸丁酯(14)、臭矢菜素A(15)。结论:除化合物8外,其余14个化合物均为首次从该植物中分离得到。细胞毒活性结果表明化合物5~11对人类白血病细胞株HL-60、肺腺癌细胞株A-549、肝癌细胞株SMMC-7721、结肠癌细胞株SW-480、乳腺癌细胞株MCF-7的体外生长具有较好的抑制作用。Objective:To study the chemical constituents of ethnodrug Murraya tetramera.Methods:The compounds were isolated and purified by silica gel column chromatography and Sephadex LH-20 gel column chromatography,the structures were identified by spectroscopy.The MTS method was used to analyze the tumor inhibitory and proliferation effects of the compounds.Results:Fifteen compounds were isolated from the ethyl acetate extraction site of alcohol extract of Murraya tetramera and identified as tricin-7-O-β-D-glucoside(1),6-methoxy coumarin-7-O-β-D-pyranglycosidase(2),N-acetyl glutamic acid(3),salvitin(4),harmine(5),piperumbellactam A(6),salutaridine(7),murradiol(8),(Z)-2,3,5,4′-tetrahydroxy-stilbene-2-O-β-D-glycoside(9),apigenin(10),piperdardine(11),astragalin(12),β-amyrin(13),methyl protocatechuate(14),cleomiscosin A(15),respectively.Conclusion:Except compound 8,other 14 compounds are isolated from Murraya tetramera for the first time.The results of cytotoxic activity show that compounds 5 to 11 show good growth inhibition on human leukemia cell line HL-60,lung adenocarcinoma cell line A-549,liver cancer cell line SMMC-7721,colon cancer cell line SW-480 and breast cancer cell line MCF-7.

关 键 词：民族药 四数九里香 化学成分 细胞毒活性 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]