银黄含片对H1N1流感小鼠炎症因子表达的影响研究  

Effect of Yinhuang Buccal Tablets on Inflammatory Factors Expression in Mice with H1N1 Influenza

作　　者：苏右竹 苏芮 吴彩军 连博 刘密凤[1] 范杰[1] 王烁 刘清泉[1] Su Youzhu;Su Rui;Wu Caijun;Lian Bo;Liu Mifeng;Fan Jie;Wang Shuo;Liu Qingquan(Beijing Hospital of Tradi-tional Chinese Medicine,Capital Medical University,Beijing 100010,China)

机构地区：[1]首都医科大学北京中医医院,北京100010 [2]北京中医药大学东直门医院,北京100700

出　　处：《中国中医急症》2024年第5期809-811,815,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家中医药管理局高水平中医药重点学科建设项目(zyyzdxk-2023001);首都卫生发展科研专项(2020-2-4192)。

摘　　要：目的评价银黄含片对H1N1流感小鼠的药效,观察其对H1N1流感小鼠炎症因子表达水平的影响。方法采用流感病毒H1N1CA07滴鼻建立H1N1小鼠模型,奥司他韦组与银黄含片组分别予奥司他韦及银黄含片溶液灌胃7 d。观察小鼠肺指数及病毒指数。采用高通量液相蛋白芯片检测小鼠血清IFN-γ、IL-12p70、IL-17A、IP-10、MIP-1β、ENA-78。结果银黄含片组肺指数较模型组显著降低,模型组小鼠IL-12p70、ENA-78和IL-17A水明显降低(P<0.01),IP-10明显增高(P<0.01)。银黄含片组IFN-γ水平与MIP-1β水平较奥司他韦组均明显降低P<0.05)。结论银黄含片能够明显减少H1N1小鼠的肺损伤,并明显降低IFN-γ与MIP-1β水平,推测银黄含片很可能是通过减少T细胞的趋化,降低流感小鼠的Th1免疫应答,从而起到达到肺保护作用。Objective:To evaluate the efficacy of Yinhuang Buccal Tablet on mice with H1N1 influenza and to explore its effect on inflammatory factors in H1N1 influenza mice.Methods:H1N1 mouse model was established by nasal drip of influenza virus H1N1CA07.The control group and the observation group were given oseltamivir and Yinhuang Buccal Tablet solution respectively by gavage for 7 days.The changes of inflammatory factors includ⁃ing IFN-γ,IL-12p70,IL-17A,IP-10,MIP-1βand ENA-78 in serum of mice were detected by high throughput liquid phase protein chip.Results:The diseased lung index of Yinhuang Buccal Tablet group was significantly lower than that of model group.The IL-12p70,ENA-78 and IL-17A water in model group were significantly lower(P<0.01),and the IP-10 was significantly higher(P<0.01).IFN-γand MIP-1βlevels in the Yinhuang Buccal Tablet group were significantly lower than those of oseltamivir group(P<0.05).Conclusion:Yinhuang Buccal Tablet could significantly reduce lung injury in H1N1 mice and the levels of IFN-γand MIP-1β.It might reduce the Th1 immune response of influenza mice by reducing the chemotaxis of T cells,thus achieving lung protection.

关 键 词：流感 银黄含片 炎症因子 免疫应答 小鼠 

分 类 号：R285.5[医药卫生—中药学]