重组人金属硫蛋白Ⅲ联合创面敷料对小鼠全层皮肤缺损创面愈合的影响  

Effects of recombinant human metallothionein-Ⅲcombined with wound dressing on wound healing of full-thickness skin defects in mice

作　　者：沈鑫 孙左义 张瑞 薛玉英 Shen Xin;Sun Zuoyi;Zhang Rui;Xue Yuying(Key Laboratory of Environmental Medicine and Engineering,Ministry of Education,School of Public Health,Southeast University,Nanjing 210009,China)

机构地区：[1]东南大学公共卫生学院,环境医学工程教育部重点实验室,南京210009

出　　处：《中华烧伤与创面修复杂志》2024年第5期425-432,共8页Chinese Journal of Burns And Wounds

基　　金：国家自然科学基金面上项目(82273674)。

摘　　要：目的探讨重组人金属硫蛋白Ⅲ(rh-MT-Ⅲ)联合创面敷料在小鼠全层皮肤缺损创面愈合中的作用。方法该研究为实验研究。取24只6周龄美国癌症研究所小鼠,雌雄各半,在每只小鼠背部制备2个对称的圆形全层皮肤缺损创面。将小鼠按性别、体重分层随机分为在创面涂布相应的溶液的生理盐水组、敷料组、rh-MT-Ⅲ组和在创面涂布rh-MT-Ⅲ和创面敷料的混合液的联合治疗组,每组6只小鼠。伤后1~7 d,每天观察所有小鼠的活动、饮食和毛发生长变化,记录小鼠体重、创面面积并计算相对创面面积百分比。伤后7 d,取小鼠创面组织,行苏木精-伊红染色观察新生肉芽组织情况,行Masson染色观察胶原纤维形成情况,行免疫荧光染色检测细胞增殖情况(以Ki67相对荧光强度表示)和细胞凋亡情况[以原位末端标记(TUNEL)相对荧光强度表示]。以上实验样本数均为6。结果伤后7 d内,4组小鼠均无活动、饮食或毛发生长异常。伤后1~7 d,4组小鼠体重总体比较,差异均无统计学意义(P>0.05)。联合治疗组小鼠伤后2、3、4、5、6、7 d相对创面面积百分比均明显低于生理盐水组、rh-MT-Ⅲ组(P<0.05),伤后3、4、5、6、7 d相对创面面积百分比均明显低于敷料组(P<0.05)。敷料组小鼠伤后6、7 d及rh-MT-Ⅲ组小鼠伤后7 d相对创面面积百分比均明显低于生理盐水组(P<0.05)。伤后7 d,联合治疗组小鼠创面组织中可见大量毛细血管和成纤维细胞,且创面上缘新生上皮组织生长优于其他3组;联合治疗组小鼠创面组织中胶原纤维致密程度较其他3组更高且排列更为有序。伤后7 d,敷料组、rh-MT-Ⅲ组、联合治疗组小鼠创面组织中Ki67相对荧光强度分别为(289±35)%、(197±17)%、(389±56)%,均明显高于生理盐水组的(100±15)%(P<0.05),联合治疗组小鼠创面组织中Ki67相对荧光强度明显高于敷料组、rh-MT-Ⅲ组(P值均<0.05)。伤后7 d,敷料组、rh-MT-Ⅲ组Objective To investigate the effects of recombinant human metallothionein-Ⅲ(rh-MT-Ⅲ)combined with wound dressing on wound healing of full-thickness skin defects in mice.Methods This study was an experimental study.Twenty-four half male and half female 6 weeks old Institute of Cancer Research mice were taken,and two symmetrical round full-thickness skin defect wounds were prepared on the back of each mouse.The mice were stratified and randomly divided into normal saline group,dressing group,rh-MT-Ⅲgroup(applying the corresponding solution on the wounds),and combined treatment group(applying a mixture of rh-MT-Ⅲand wound dressing on the wounds)according to sex and body weight,with 6 mice in each group.From 1 to 7 d after injury,all mice were observed daily for changes in activity,diet,and fur growth,their body weights and wound areas were recorded,and the relative wound area percentages were calculated.On 7 d after injury,the wound tissue of mice was taken for hematoxylin-eosin staining to observe the newborn granulation tissue,for Masson staining to observe collagen fiber formation,and for immunofluorescence staining to detect cell proliferation(denoted as Ki67 relative fluorescence intensity)and cell apoptosis(denoted as TdT-mediated dUTP nick end labeling(TUNEL)relative fluorescence intensity).The sample size in the above experiments was 6.Results There were no abnormalities in activity,diet,or fur growth in the 4 groups of mice within 7 d after injury.There were no statistically significant differences in the overall comparison of the body weights of mice in the 4 groups from 1 to 7 d after injury(P>0.05).The relative wound area percentages of mice in combined treatment group were significantly lower than those in normal saline group and rh-MT-Ⅲgroup on 2,3,4,5,6,and 7 d after injury(P<0.05),and the relative wound area percentages of mice in combined treatment group were significantly lower than those in dressing group on 3,4,5,6,and 7 d after injury(P<0.05).The relative wound area percentages of mice

关 键 词：创伤和损伤 金属硫蛋白 生物敷料 细胞增殖 细胞凋亡 创面修复 

分 类 号：R641[医药卫生—外科学]