淫羊藿总黄酮胶囊对脑卒中后认知障碍大鼠的作用  

Effects of epimedium total flavone capsules on post-stroke cognitive impairment in rats

作　　者：杨沛勋 范小雪 刘忞璇 刘婧 曹亮[1,2,3] 王振中 王欣 张新庄[2,3] 肖伟 YANG Pei-xun;FAN Xiao-xue;LIU Min-xuan;LIU Jing;CAO Liang;WANG Zhen-zhong;WANG Xin;ZHANG Xin-zhuang;XIAO Wei(Nanjing University of Chinese Medicine,Nanjing 210023,China;National Key Laboratory on Technologies for Chinese Medicine Pharmaceutical Process Control and Intelligent Manufacture,Lianyungang 222001,China;Jiangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China)

机构地区：[1]南京中医药大学,江苏南京210023 [2]中药制药过程控制与智能制造技术全国重点实验室,江苏连云港222001 [3]江苏康缘药业股份有限公司,江苏连云港222001

出　　处：《中国中药杂志》2024年第8期2262-2272,共11页China Journal of Chinese Materia Medica

基　　金：国家中医药管理局“岐黄学者”中医药领军人才项目。

摘　　要：探究淫羊藿总黄酮胶囊对脑卒中后认知障碍(post-stroke cognitive impairment,PSCI)大鼠的作用。构建大鼠大脑中动脉短暂性缺血(transient middle cerebral artery occlusion,tMCAO)模型,将出现神经功能受损的大鼠随机分为模型对照组,淫羊藿总黄酮胶囊低、中、高剂量组,尼莫地平片组。给药后检测大鼠认知功能,苏木精-伊红(hematoxylin-eosin,HE)染色观察脑组织病理变化,免疫荧光法检测脑组织中神经元细胞标记物(neuronal nuclei,NeuN)和星形胶质细胞标记物(glial fibrillary acidic protein,GFAP)表达分布情况;检测大鼠血清中β淀粉样蛋白1-42(amyloid beta 1-42,Aβ_(1-42))、神经元烯醇化酶(neuron specific enolase,NSE)、乙酰胆碱(acetylcholine,ACH)、多巴胺(dopamine,DA)、5-羟色胺(5-hydroxytryptamine,5-HT)、去甲肾上腺素(norepinephrine,NE)、白细胞介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和超敏C反应蛋白(hypersensitive C-reactive protein,hs-CRP)的含量。另外,采用Western blot检测海马组织中核转录因子-κB(nuclear factor-kappaB,NF-κB)、p-NF-κB、NF-κB抑制蛋白α(alpha inhibitor of NF-κB,IκBα)、p-IκBα蛋白的表达量。实验结果显示,淫羊藿总黄酮胶囊可改善模型大鼠的认知功能,其作用机制可能是通过调节p-IκBα和p-NF-κB蛋白的表达,进而抑制缺血再灌注引起的炎症反应。To investigate the effect of epimedium total flavone capsules on post-stroke cognitive impairment(PSCI)in rats.The transient middle cerebral artery occlusion(tMCAO)model was constructed on selected rats,and rats with impaired neurological function were randomly divided into the model group,low,middle,and high dose groups of epimedium total flavone capsules,and nimodipine tablet group.The cognitive function of rats was measured after administration.Pathological changes in brain tissue were observed after hematoxylin-eosin staining(HE).Neuronal nuclei(NeuN)and glial fibrillary acidic protein(GFAP)distribution in brain tissue were tested by immunofluorescent staining.The level of amyloid beta 1-42(Aβ_(1-42)),neuron specific enolase(NSE),acetylcholine(ACH),dopamine(DA),5-hydroxytryptamine(5-HT),norepinephrine(NE),interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),and hypersensitive C-reactive protein(hs-CRP)in rat serum was tested.Moreover,Western blot was utilized to test the expression of nuclear factor-kappaB(NF-κB),p-NF-κB,alpha inhibitor of NF-κB(IκBα)protein,and p-IκBαprotein in the hippocampus.The experimental results showed that epimedium total flavone capsules can improve the cognitive function of model rats,and the mechanism may be related to the regulation of the expression of p-IκBαand p-NF-κB proteins,so as to inhibit inflammatory response induced by ischemia-reperfusion.

关 键 词：淫羊藿总黄酮胶囊 卒中后认知障碍 IκBα/NF-κB 炎症反应 

分 类 号：R285.5[医药卫生—中药学]