Tissue adhesive,ROS scavenging and injectable PRP-based‘plasticine’for promoting cartilage repair  

作　　者：Shiao Li Dawei Niu Haowei Fang Yancheng Chen Jinyan Li Kunxi Zhang Jingbo Yin Peiliang Fu 

机构地区：[1]Department of Orthopedics,Shanghai Changzheng Hospital,Naval Medical University,Shanghai 200003,P.R.China [2]Department of Polymer Materials,School of Materials Science and Engineering,Shanghai University,Shanghai 200444,P.R.China

出　　处：《Regenerative Biomaterials》2024年第1期94-107,共14页再生生物材料（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos 82272472,52373146,52173131,51973108);Natural Science Foundation of Shanghai(Grant No.22ZR1424700).

摘　　要：Platelet-rich plasma(PRP)that has various growth factors has been used clinically in cartilage repair.However,the short residence time and release time at the injury site limit its therapeutic effect.The present study fabricated a granular hydrogel that was assembled from gelatin microspheres and tannic acid through their abundant hydrogen bonding.Gelatin microspheres with the gelatin concentration of 10wt%and the diameter distribution of 1-10 lm were used to assemble by tannic acid to form the granular hydrogel,which exhibited elasticity under low shear strain,but flowability under higher shear strain.The viscosity decreased with the increase in shear rate.Meanwhile,the granular hydrogel exhibited self-healing feature during rheology test.Thus,granular hydrogel carrying PRP not only exhibited well-performed injectability but also performed like a‘plasticine’that possessed good plasticity.The granular hydrogel showed tissue adhesion ability and reactive oxygen species scavenging ability.Granular hydrogel carrying PRP transplanted to full-thickness articular cartilage defects could integrate well with native cartilage,resulting in newly formed cartilage articular fully filled in defects and well-integrated with the native cartilage and subchondral bone.The unique features of the present granular hydrogel,including injectability,plasticity,porous structure,tissue adhesion and reactive oxygen species scavenging provided an ideal PRP carrier toward cartilage tissue engineering.

关 键 词：cartilage regeneration granular hydrogel GELATIN tannic acid PRP 

分 类 号：R318[医药卫生—生物医学工程]