甘肃口岸出入境人员乙肝病毒和丁肝病毒混合感染调查及乙肝病毒基因型分析  

Analysis of HBV and HDV coinfection and HBV genotyping among entry-exit population at Gansu port

作　　者：王莉 王姣乐 宋晓芸 赵志亮 巩伟兵 高云新 张妙婷 张红霞 刘芳 高晓强 WANG Li;WANG Jiaoyue;SONG Xiaoyun;ZHAO Zhiliang;GONG Weibing;GAO Yunxin;ZHANG Miaoting;ZHANG Hongxia;LIU Fang;GAO Xiaoqiang(Gansu International Trawel Healthcare Center(Lanzhou Customs Port Outpatient Departemnt),Lanzhou,Gansu730020,China)

机构地区：[1]甘肃国际旅行卫生保健中心(兰州海关口岸门诊部),甘肃兰州730020

出　　处：《中国国境卫生检疫杂志》2024年第2期116-120,共5页Chinese Journal of Frontier Health and Quarantine

基　　金：兰州海关科研项目(LK-2022-009)。

摘　　要：目的了解甘肃口岸出入境人员乙肝病毒(HBV)和丁肝病毒(HDV)混合感染情况及乙肝病毒基因分型,为甘肃口岸乙肝和丁肝的预防控制提供科学依据。方法收集2017—2022年在甘肃国际旅行卫生保健中心进行健康体检的出入境人员HBsAg阳性血清样本,用酶联免疫法和PCR法分别检测HDV抗体和HBV S基因片段。用NCBI在线病毒分型工具进行HBV基因分型,用Mega 11软件构建进化树确定基因亚型。结果共收集出入境人员HBsAg阳性血清样本672人份,检出HDV抗体阳性1例,HBV DNA阳性322例。HBV基因分型成功118例,其中B基因型11例、C基因型51例、D基因型55例、E基因型1例,B基因型分为B1、B2、B4和B6亚型,B2亚型7例,B4亚型2例,B1亚型和B6亚型各1例;C基因型分为C1、C2和C5亚型,C2亚型42例,C1亚型6例,C5亚型3例;D基因型分为D1、D3亚型和C/D重组基因型,C/D重组基因型44例,D1亚型7例,D3亚型4例。结论甘肃口岸出入境人群HBV和HDV混合感染率低,HBV优势基因型为C型和D型,亚型以C2亚型和C/D重组基因型为主。Objective To investigate the coinfection of HBV and HDV,detect hepatitis B virus genotypes among entry-exit personnel in Gansu port,then provide a scientific basis for the prevention and control of hepatitis B and hepatitis D at Gansu port.Methods The HBsAg positive serum samples of entry-exit people who took health examination at Gansu International Travel Healthcare Center from 2017 to 2022 were collected,HDV antibody and HBV S gene partial sequence were detected by ELISA and PCR.HBV genotypes were performed using the virus typing tool of NCBI online,and subgenotypes were determined by Mega 11 software to construct evolutionary trees.Results In 2017-2022,a total of 672 HBsAg positive serum samples were collected from entry-exit people who took health examination in Gansu International Travel Healthcare Center.One case of HDV antibody positive and322 cases of HBV DNA positive were detected.118 cases of HBV DNA positive were genotyped successfully,11cases were genotype B,51 cases were genotype C,55 cases were genotype D,and 1 was type E.Among the genotype B,7,2,1,1 cases were B2,B4,B1 and B6 subtype.Including 42 cases of C2 subtype,6 cases of C1 and 3cases of C5 in genotype C.Among the genotype D,44,7,4 cases were C/D recombinant genotype,D1 and D3subtype.Conclusion The entry-exit population in Gansu port,the mixed infection rate of HBV and HDV was low.The dominant genotypes of HBV were C and D,the subtypes were mainly C2 subtype and C/D recombinant genotype.

关 键 词：乙肝病毒 丁肝病毒 基因分型 出入境人员 

分 类 号：R183[医药卫生—流行病学]