二穗短柄草(Brachypodium distachyon)BdCO基因调控开花的转录组学分析  

Transcriptome Analysis of BdCO Gene Controlling Flowering in Brachypodium distachyon

作　　者：杨龙姣 路雪萍 童伟杨 马培杰[2] 罗文举 陈才俊[2] 王小利[2] YANG Longjiao;LU Xueping;TONG Weiyang;MA Peijie;LUO Wenju;CHEN Caijun;WANG Xiaoli(College of Animal Sciences,Guizhou University,Guiyang,550025;Guizhou Institute of Prataculture,Guizhou Academy of Agricultural Sciences,Guiyang,550006;Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),College of Life Sciences/Institute of Agro-bioengineering,Guizhou University,Guiyang,550025)

机构地区：[1]贵州大学动物科学学院,贵阳550025 [2]贵州省农业科学院草业研究所,贵阳550006 [3]贵州大学生命科学学院/农业生物工程研究院,山地植物资源保护与种质创新教育部重点实验室,贵阳550025

出　　处：《基因组学与应用生物学》2024年第4期602-615,共14页Genomics and Applied Biology

基　　金：国家自然科学基金项目(32060394);科研机构创新能力建设专项(黔科合服企[2022]004);贵州省基金项目(黔科合基础-ZK[2023]一般164);院国基后补助/黔农科院国基后补助(2021)54号及院国基后补助/黔农科院国基后补助(2021)33号共同资助。

摘　　要：CONSTANS(CO)是植物光周期诱导开花途径中的关键基因之一。为探究BdCO在光周期途径中的分子调控机制,本研究对野生型二穗短柄草(Brachypodium distachyon)Bd21植株、过表达BdCO基因型二穗短柄草(CO_OX)植株和BdCO基因敲除型二穗短柄草(CO_A3)植株进行转录组测序分析,对差异表达基因进行GO和KEGG功能富集分析,最后观察三种植株的开花表型。结果表明,对比Bd21 vs CO_OX和Bd21 vs CO_A3的基因表达量,分别检测到1382个和773个差异表达基因;GO功能富集分析发现,Bd21 vs CO_OX的差异表达基因主要富集在小核仁核糖核蛋白复合物、snoRNA结合和rRNA处理中,Bd21 vs CO_A3的差异表达基因主要富集在类囊体、色素结合和光合作用中;KEGG通路富集分析发现,Bd21 vs CO_OX的差异表达基因主要富集在植物激素信号转导、真核生物中的核糖体生物发生、光合作用-天线蛋白和昼夜节律-植物等通路,Bd21 vs CO_A3的差异表达基因主要富集在MAPK信号通路-植物、真核生物中的核糖体生物发生和光合作用-天线蛋白等通路;在长日照条件下,CO_OX植株的开花时间比野生型Bd21的提前约1.90 d,CO_A3植株的开花时间比野生型Bd21的延迟约7.92 d。综上,BdCO影响光周期途径相关基因的表达,同时影响二穗短柄草的开花时间,说明BdCO在二穗短柄草的光周期途径调控开花的过程中发挥重要作用。The CONSTANS(CO) is one of the key genes in the photoperiodic flowering pathway of plants. To explore the molecular regulatory mechanism of BdCO in the photoperiodic pathway, this study conducted transcriptome sequencing analysis on the wild-type Brachypodium distachyon Bd21 plants, the overexpressing BdCO genotype B.distachyon(CO_OX) plants, and the BdCO knockout B. distachyon(CO_A3) plants. Differential gene expression analysis was performed, and GO and KEGG functional enrichment analyses were conducted on the differentially expressed genes. Finally, the flowering phenotypes of the three types of plants were observed. The results showed that compared to Bd21 vs CO_OX and Bd21 vs CO_A3, 1 382 and 773 differentially expressed genes were detected, respectively. The GO functional enrichment analysis revealed that the differentially expressed genes in Bd21 vs CO_OX were mainly enriched in small nucleolar ribonucleoprotein complex, snoRNA binding and rRNA processing, while those in Bd21 vs CO_A3 were mainly enriched in thylakoid, pigment binding, and photosynthesis. The KEGG pathway enrichment analysis showed that the differentially expressed genes in Bd21 vs CO_OX were mainly enriched in plant hormone signal transduction, ribosome biogenesis in eukaryotes, photosynthesis-antenna proteins and circadian rhythm-plant, while those in Bd21 vs CO_A3 were mainly enriched in MAPK signaling pathway-plant, ribosome biogenesis in eukaryotes and photosynthesis-antenna proteins. Under long-day conditions, the flowering time of CO_OX plants was about 1.90 days earlier than that of Bd21 plants, while the flowering time of CO_A3 plants was delayed by about 7.92 days compared to Bd21 plants.In conclusion, BdCO affects the expression of genes related to the photoperiodic pathway and also influences the flowering time of B.distachyon, indicating that BdCO plays an important role in the regulation of flowering in the photoperiodic pathway of B.distachyon.

关 键 词：二穗短柄草 BdCO基因 转录组学 光周期 

分 类 号：Q943.2[生物学—植物学]