玉米蚜线粒体基因组测序及蚜族系统发育分析  

Sequencing of the Mitochondrial Genome of Rhopalosiphum maidis and Phylogenetic Analysis of Aphidini

作　　者：林兴雨 任凯杰 宋凡 宋南[1] 李虎 LIN Xingyu;REN Kaijie;SONG Fan;SONG Nan;LI Hu(Plant Protection College,Henan Agricultural University,Zhengzhou,450046;College of Plant Protection,China Agricultural University,Beijing,100193)

机构地区：[1]河南农业大学植物保护学院,郑州450046 [2]中国农业大学植物保护学院,北京100193

出　　处：《基因组学与应用生物学》2024年第4期675-684,共10页Genomics and Applied Biology

基　　金：国家自然科学基金项目(U1904104)资助。

摘　　要：为探究玉米蚜(Rhopalosiphum maidis)线粒体基因组结构特征及蚜族(Aphidini)的系统发育关系,本研究利用高通量测序技术测定了玉米蚜线粒体基因组全序列。选择已知的蚜族18个物种线粒体基因组作为内群,选择长管蚜族(Macrosiphini)2个物种线粒体基因组作为外群,利用最大似然法(maximum likelihood method)和贝叶斯法(Bayesian inference method)重建蚜族的系统发育关系。玉米蚜的线粒体基因组全长为15096 bp,呈环状,包括13个蛋白质编码基因(protein-coding genes)、22个tRNA基因和2个rRNA基因,还有一段非编码控制区。13个蛋白质编码基因的起始密码子全为ATN;3个蛋白质编码基因cox1、nad4和nad5具有不完整的终止密码子T或TA,其余10个蛋白质编码基因为完整的终止密码子TAA或TAG。本研究利用线粒体基因组数据重建了蚜族的系统发育关系,最大似然法和贝叶斯法构建的蚜族的系统发育关系是基本一致的。其中,色蚜属(Melanaphis)、桃粉蚜属(Hyalopterus)、蚜属(Aphis)为单系群,缢管蚜属(Rhopalosiphum)为非单系群。To investigate the structural features of the mitochondrial genome of Rhopalosiphum maidis and its phylogenetic relationship within Aphidini,high-throughput sequencing technology was utilized to determine the complete sequence of the mitochondrial genome in Rhopalosiphum maidis in this study.In phylogenetic analyses,the mitochondrial genomes of 18 known Aphidini species were selected as the ingroup,while the mitochondrial genomes of 2 Macrosiphini species were selected as the outgroup.The phylogenetic relationships of Aphidini was reconstructed by using maximum likelihood method and Bayesian inference method.The mitochondrial genome of Rho-palosiphum maidis was a circular molecule of 15096 bp in length,which contained 13 protein-coding genes,22 tRNA genes,2 rRNA genes and a non-coding control region.All 13 protein-coding genes started with the codons of ATN.While 3 protein-coding genes cox1,nad4 and nad5 employed the incomplete T or TA as the stop codon,the remaining 10 protein-coding genes terminated with TAA or TAG.In this research,the phylogenetic relationships among the Aphidini were reconstructed by utilizing mitochondrial genome data,revealing that the phylogenetic trees constructed by the maximum likelihood method and Bayesian inference method are essentially congruent.Among them,the genus Melanaphis,Hyalopterus and Aphis were monophyletic groups,while the genus Rhopalosiphum was non-monophyletic group.

关 键 词：玉米蚜 缢管蚜属 蚜族 线粒体基因组 系统发育 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治]