南海软珊瑚Lobophytum sp.中二萜类化合物的研究  

Studies on diterpenoids of soft coral Lobophytum sp.from South China Sea

作　　者：周康 刘冬云 王斌 程伟[2] ZHOU Kang;LIU Dongyun;WANG Bin;CHENG Wei(College of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316022,China;State Key Laboratory of Natural and Biomimetic Medicines,Peking University,Beijing 100191,China)

机构地区：[1]浙江海洋大学食品与药学学院,浙江舟山316022 [2]北京大学天然药物及仿生药物国家重点实验室,北京100191

出　　处：《中国海洋药物》2024年第2期60-66,共7页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金重大项目(81991521)资助。

摘　　要：目的对海洋来源软珊瑚Lobophytum sp.中的化学成分结构多样性及生物活性进行研究。方法利用硅胶、十八烷基硅烷键合硅胶(ODS-C18)、凝胶Sephadex LH-20和半制备高效液相(semi-HPLC)等色谱学方法对软珊瑚乙酸乙酯提取物中的化学成分进行分离;利用紫外(UV)、核磁共振波谱(NMR)、质谱(MS)等波谱学方法,结合与文献的数据对比确定化合物的结构。结果从软珊瑚的乙酸乙酯提取物中分离鉴定了10个西松烷型二萜类化合物,分别为sarcomililatin B(1)、lobophytin A(2)、(-)-isosarcophine(3)、(+)-sarcophytoxide(4)、isosarcophytoxide(5)、(+)-11,12-epoxy-11,12-dihydrocembrene-C(6)、cherbonnolide B(7)、sarcomililatins A(8)、sarcophytonin B(9)和3,4-dihydro-4α-hydroxy-△^(2)-sarcophine(10)。其中化合物7和8为首次从该属软珊瑚中分离得到。化合物1和3在20μmol/L物质的量浓度下对脂多糖(LPS)诱导NO产生的抑制率分别为31.5%和28.6%。结论从南海软珊瑚Lobophytum sp.中分离得到了化合物1~10,其中化合物1和3对LPS诱导NO产生具有一定的抑制作用。Objective To investigate the structural diversity and biological activity of chemical constituents from soft coral lobophytum sp..Methods The compounds were isolated from crude extract and purified by silica gel column,ODS-C18 column,Sephadex LH-20 and HPLC.Their structures were determined by analysis of UV,NMR,MS data and comparison with those reported in literatures.Results Ten cembrane diterpenoids were isolated and identified as sarcomililatin B(1),lobophytin A(2),(-)-isosarcophine(3),(+)-sarcophytoxide(4),isosarcophytoxide(5),(+)-11,12-epoxy-11,12-dihydrocembrene-C(6),cherbonnolide B(7),sarcomililatins A(8),sarcophytonin B(9)and 3,4-dihydro-4α-hydroxy-△^(2)-sarcophine(10).Compounds 7 and 8 were isolated from this genus for the first time.At a concentration of 20μmol/L,compounds 1 and 3 exhibited inhibition of LPS-induced NO production with inhibitory rates as 31.5%and 28.6%,respectively.Conclusion Ten cembrane diterpenoids were isolated from soft corals Lobophytum sp..Compounds 1 and 3 showed inhibition effects on NO production induced by LPS.

关 键 词：软珊瑚 次级代谢产物 西松烷型二萜 抗炎活性 Lobophytum sp. 

分 类 号：R931.77[医药卫生—生药学]