Estimation-free spatial-domain image reconstruction of structured illumination microscopy  

作　　者：Xiaoyan Li Shijie Tu Yile Sun Yubing Han Xiang Hao Cuifang kuang Xu Liu 

机构地区：[1]State Key Laboratory of Extreme Photonics and Instrumentation College of Optical Science and Engineering,Zhejiang University Hangzhou 310027,P.R.China [2]ZJU-Hangzhou Global Scientific and Technological Innovation Center Hangzhou 311200,P.R.China [3]Collaborative Innovation Center of Extreme Optics,Shanxi University Taiyuan,Shanxi 030006,P.R.China [4]A dvanced Biomedical Imaging Facility-Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology Wuhan,Hubei 430074,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2024年第2期45-58,共14页创新光学健康科学杂志（英文）

基　　金：funded by the National Natural Science Foundation of China(62125504,61827825,and 31901059);Zhejiang Provincial Ten Thousand Plan for Young Top Talents(2020R52001);Open Project Program of Wuhan National Laboratory for Optoelectronics(2021WNLOKF007).

摘　　要：Structured illumination microscopy(SIM)achieves super-resolution(SR)by modulating the high-frequency information of the sample into the passband of the optical system and subsequent image reconstruction.The traditional Wiener-filtering-based reconstruction algorithm operates in the Fourier domain,it requires prior knowledge of the sinusoidal illumination patterns which makes the time-consuming procedure of parameter estimation to raw datasets necessary,besides,the parameter estimation is sensitive to noise or aberration-induced pattern distortion which leads to reconstruction artifacts.Here,we propose a spatial-domain image reconstruction method that does not require parameter estimation but calculates patterns from raw datasets,and a reconstructed image can be obtained just by calculating the spatial covariance of differential calculated patterns and differential filtered datasets(the notch filtering operation is performed to the raw datasets for attenuating and compensating the optical transfer function(OTF)).Experiments on reconstructing raw datasets including nonbiological,biological,and simulated samples demonstrate that our method has SR capability,high reconstruction speed,and high robustness to aberration and noise.

关 键 词：Structured illumination microscopy image reconstruction spatial domain digital micromirror device(DMD) 

分 类 号：TP391.41[自动化与计算机技术—计算机应用技术] TH742[自动化与计算机技术—计算机科学与技术]