Three-dimensional morphological and fluorescent imaging of zebrafish with a continuous-rotational light-sheet microscope  

作　　者：Dongmin Zhang Guang Yang Yao Tan Chong Chen Jie Zhang Hui Li 

机构地区：[1]School of Biomedical Engineering(Suzhou)Division of Life Sciences and Medicine University of Science and Technology of China Hefei 230041,P.R.China [2]Suzhou Institute of Biomedical Engineering and Technology Chinese A cademy of Sciences Suzhou,Jiangsu 215163,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2024年第2期59-66,共8页创新光学健康科学杂志（英文）

基　　金：the National Natural Science Foundation of China(62205368);the Key Research and Development Program of Jiangsu Province(BE2020664).

摘　　要：Light-sheet fluorescence microscopy(LSFM)has been widely used to image the three-dimensional(3D)structures and functions of various millimeter-size bio-specimen such as zebrafish.However,the sample adsorption and scattering cause shading of the light-sheet illumination,preventing the even 3D image of thick samples.Herein,we report a continuous-rotational light-sheet microscope(CR-LSM)that enables simultaneous 3D bright-field and fluorescence imaging.With a high-accuracy rotational stage,CR-LSM records the outline projections and the fluorescent images of the sample at multiple rotation angles.Then,3D morphology and fluorescent structure were reconstructed with a developed algorithm.Using CR-LSM,zebrafish’s whole-fish contour and blood vessel structures were obtained simultaneously.

关 键 词：Light-sheet microscope zebrafish blood vessels MORPHOLOGY 

分 类 号：TH742[机械工程—光学工程]