Tissue optical clearing enhances efficacy of vascular targeted photodynamic therapy of mouse dorsal skin  

作　　者：Ying Liu Qiushi Wang Yidi Liu Ying Wang Haixia Qiu Dan Zhu Ying Gu Defu Chen 

机构地区：[1]School of Optics and Photonics,Beijing Institute of Technology,Beijing 100081,P.R.China [2]School of Medical Technology,Beijing Institute of Technology,Beijing 100081,P.R.China [3]Department of Laser Medicine,First Medical Center of PLA General Hospital,Beijing 100853,P.R.China [4]Britton Chance Center for Biomedical Photonics-MoE Key Laboratory for Biomedical Photonics,A duanced Biomedical Imaging Facility,Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan 430074,Hubei,P.R.China [5]Precision Laser Medical Diagnosis and Treatment Innovation Unit,Chinese A cademy of Medical Sciences,Beijing 100000,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2024年第2期67-78,共12页创新光学健康科学杂志（英文）

基　　金：supported by the National Natural Science Foundation of China(Grant Numbers 62205025 and 61835015);Beijing Natural Science Foundation(7222309);the Open Project Program of Wuhan National Laboratory for Optoelectronics(2020WNLOKF025);CAMS Innovation Fund for Medical Sciences(CIFMS)(2019-I2M-5-061);Beijing Institute of Technology Research Fund Program for Young Scholars(XSQD-202123001).

摘　　要：Vascular-targeted photodynamic therapy(V-PDT)is an effective treatment for port wine stains(PWS).However,repeated treatment is usually needed to achieve optimal treatment outcomes,possibly due to the limited treatment light penetration depth in the PWS lesion.The optical clearing technique can increase light penetration in depth by reducing light scattering.This study aimed to investigate the V-PDT in combination with an optical clearing agent(OCA)for the therapeutic enhancement of V-PDT in the rodent skinfold window chamber model.Vascular responses were closely monitored with laser speckle contrast imaging(LSCI),optical coherence tomography angiography,and stereo microscope before,during,and after the treatment.We further quantitatively demonstrated the effects of V-PDT in combination with OCA on the blood flow and blood vessel size of skin microvasculature.The combination of OCA and V-PDT resulted in significant vascular damage,including vasoconstriction and the reduction of blood flow.Our results indicate the promising potential of OCA for enhancing V-PDT for treating vascular-related diseases,including PWS.

关 键 词：Vascular-targeted photodynamic therapy(V-PDT) optical clearing agent(OCA) treatment efficacy enhancement skin-fold window chamber port wine stains 

分 类 号：O43[机械工程—光学工程]