多花黄精中果聚糖产物的抗疲劳与改善肠道菌作用研究  

Study on the Anti-Fatigue and Intestinal Flora Improvement Effects of Fructan Products Isolated from Polygonatum Cyrtonema Hua

作　　者：王娜 刘涛 马畅 虞晓弦 嵇晶[1,2] 王令充 程建明[1,2] WANG Na;LIU Tao;MA Chang;YU Xiaoxian;JI Jing;WANG Lingchong;CHENG Jianming(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Province Engineering Research Center of Classical Prescription,Nanjing 210023,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏省经典名方工程技术研究中心,江苏南京210023

出　　处：《南京中医药大学学报》2024年第5期494-502,共9页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：南京中医药大学-艾兰得健康产品研发项目(2021-003)。

摘　　要：目的研究多花黄精精制多糖的结构特征并探究其抗疲劳及改善肠道菌作用,为以多花黄精精制多糖为功能性成分的产品开发提供理论依据。方法采用PMP柱前衍生化-高效液相色谱法、三甲基硅醚衍生化-气相色谱法等方法对多花黄精精制多糖进行化学表征;采用小鼠负重游泳试验结合生化指标评价其抗疲劳作用;采用16S rRNA肠道菌群测序研究多花黄精精制多糖的改善肠道菌作用。结果获得的多花黄精精制多糖是一种果聚糖产物,且含有少量葡萄糖(Glc)、半乳糖(Gal)、阿拉伯糖(Ara)、甘露糖(Man)、鼠李糖(Rha)和半乳糖醛酸(GalA),其相对分子质量在0.4~285 kDa内,主要组分的分子量在3 kDa左右。与模型组比较,多花黄精精制多糖高剂量组能明显延长小鼠的力竭游泳时间(P<0.01),降低血清中BUN、UA水平,显著降低肌肉组织中LA(P<0.01)和MDA水平(P<0.05);显著提高肝脏组织中SOD(P<0.01)活性,GSH-Px(P<0.001)和CAT(P<0.05)水平。与空白对照组相比,多花黄精精制多糖低剂量组能够上调小鼠粪便微生物中益生菌伊格尔兹氏菌属(g_unclassified_f_Eggerthelaceae)和魏斯氏菌属(g_Weissella)相对丰度(P<0.05)。结论确定了多花黄精精制多糖的化学组成,并证明了该多糖具有较好的抗疲劳功效,能促进肠道中益生菌生长,为以多花黄精精制多糖作为功能性成分的产品开发提供依据。OBJECTIVE To study the structural characteristics of purified polysaccharide isolated from Polygonatum cyrtonema Hua and explore its anti-fatigue and intestinal bacteria improvement effects,so as to provide theoretical basis for the development of products using this polysaccharide as functional ingredients.METHODS PMP pre-column derivatization-high performance liquid chromatography,trimethylsilyl ether derivatization-gas chromatography and other methods were used to chemically characterize the purified polysaccharide;the mouse weight-loading swimming test and biochemical indicators were used to evaluate its anti-fatigue effect;16S rRNA intestinal flora sequencing was adopted to study the effect of the purified product in improving intestinal bacteria.RESULTS The obtained polysaccharide was a fructan product and contained small amounts of glucose(Glc),galactose(Gal),arabinose(Ara),mannose(Man),rhamnose(Rha)and galacturonic acid(GalA),which had a relative molecular mass in the range of 0.4-285 kDa,and the molecular weight of the main component was around 3 kDa.Compared with the model group,the high-dose polysaccharide group could significantly prolong the exhaustive swimming time of mice(P<0.01),reduce the levels of BUN and UA in serum and the LA(P<0.01)and MDA levels(P<0.05)in muscle tissue,and enhance SOD(P<0.01),GSH-Px(P<0.001)and CAT(P<0.05)levels in liver tissue.Compared with the blank control group,the low-dose polysaccharide group could increase the relative abundance of probiotics Eggerthelaceae(g_unclassified_f_Eggerthelaceae)and Weissella(g_Weissella)in the fecal microorganisms of mice(P<0.05).CONCLUSION The chemical composition of purified polysaccharide isolated from Polygonatum cyrtonema Hua is determined,and it is proven that the polysaccharide has a good anti-fatigue effect and can promote the growth of probiotics in the intestine,which provides a basis for product development using this polysaccharide as a functional ingredient.

关 键 词：多糖 多花黄精 抗疲劳活性 结构表征 肠道菌群 

分 类 号：R284[医药卫生—中药学]