青海牧区犊牛腹泻大肠埃希氏菌毒力基因及血清型分布特征研究  

Analysis of Virulence Genes and Serotype Distribution Characteristics of E.coli Causing Calf Diarrhea in Pastoral Area of Qinghai Plateau

作　　者：石田 韩生义 李淑萍 胡国元[2,3] 李玲霞 李生庆 SHI Tian;HAN Sheng-yi;LI Shu-ping;HU Guo-yuan;LI Ling-xia;LI Sheng-qing(College of Agriculture and Animal Husbandry,Qinghai University,Xining,Qinghai,810016,China;College of Animal Science and Veterinary Medicine,Qinghai University,Xining,Qinghai,810016,China;Qinghai Provincial Key Laboratory of Animal Disease Pathogen Diagnosis and Green Prevention and Control Technology,Xining,Qinghai,810016,China)

机构地区：[1]青海大学农牧学院,青海西宁810016 [2]青海大学畜牧兽医科学院,青海西宁810016 [3]青海省动物疾病病原诊断与绿色防控技术研究重点实验室,青海西宁810016

出　　处：《动物医学进展》2024年第6期20-25,共6页Progress In Veterinary Medicine

基　　金：青海省科学技术厅科技成果转化专项项目(2022-NK-118)。

摘　　要：为掌握青海牧区致牦牛腹泻大肠埃希氏菌病的流行特征,以期为牦牛大肠埃希氏菌性腹泻的预防和治疗提供依据,对青海省玉树、果洛、海北地区采集的99份犊牦牛腹泻相关样本进行致病性大肠埃希氏菌的分离培养及O抗原血清分型、毒力基因扩增及小鼠毒性试验等研究。结果显示,分离菌株主要携带F17、ompA、fimC、espA、HPI、LEE、VT1、SLT2等毒力基因,未检出K88、K99、LT1等毒力基因,小鼠最低致死剂量为2.5×10^(9)CFU/mL,优势血清型为O25、O55和O107,分别占定型血清的25.40%、14.29%和19.05%,系统主要发育群为B1群。研究结果将为后续青海地区致犊牦牛腹泻大肠杆菌病的治疗、发病机制探讨及疫苗研制提供支撑。In order to grasp the prevalence characteristics of yak diarrhea-colibacillosis in Qinghai pastoral area,and to provide a technical basis for the prevention and treatment of yak colibacillosis,99 calf yak diarrhea samples collected in Yushu,Guoluo and Haibei areas of Qinghai province were used to isolate and culture for pathogenic E.coli,O antigen serotyping,virulence gene amplification and mouse toxicity test were conducted.The results showed that the isolated strains mainly carried F17,ompA,fimC,espA,HPI,LEE,VT1,SLT2 and other virulence genes,no virulence genes such as K88,K99 and LT1 were detected,the lowest lethal dose of mice was 2.5×10^(9)CFU/mL,and the dominant serotypes were O25,O55 and O107,accounting for 25.40%,14.29%and 19.05%of the stereotyped serotypes,respectively,and the main phylogenetic group was B1.This study provides data and theoretical support for the subsequent treatment,pathogenesis and vaccine development of yak diarrhea in Qinghai region.

关 键 词：牦牛 致病性大肠埃希氏菌 毒力基因 血清分型 系统发育 

分 类 号：S852.612[农业科学—基础兽医学] S858.23[农业科学—兽医学]