新疆某规模化肉牛场牛病毒性腹泻病毒分离与基因型鉴定  

Isolation and Genotype Identification of Bovine Viral Diarrhea Virus from A Large-scale Beef Cattle Farm in Xinjiang

作　　者：黄琴 陈红强 张怡淼 马颖超 胡建军[1,2] 张伟 HUANG Qin;CHEN Hong-qiang;ZHANG Yi-miao;MA Ying-chao;HU Jian-jun;ZHANG Wei(Engineering Laboratory for Tarim Animal Diseases Diagnosis and Control of Xinjiang Production&Construction Corps,Alar,Xinjiang,843300,China;College of Animal Science and Technology,Tarim University,Alar,Xinjiang,843300,China;Aksu Xiyu Animal Development Limited Company,Aksu,Xinjiang,843000,China)

机构地区：[1]塔里木大学兵团塔里木动物疫病诊断与防控工程实验室,新疆阿拉尔843300 [2]塔里木大学动物科学与技术学院,新疆阿拉尔843300 [3]阿克苏市西域牧业发展有限公司,新疆阿克苏843000

出　　处：《动物医学进展》2024年第7期18-23,共6页Progress In Veterinary Medicine

基　　金：兵团塔里木动物疫病诊断与防控工程实验室项目(ELDC202304);兵团科技局现代农业科技攻关与成果转化计划项目(2016AC012)。

摘　　要：新疆许多规模化牛场存在牛病毒性腹泻病毒(BVDV)的感染,且因病毒基因型具有多样性,给该病的防控、疫苗选择、净化带来了一定的困难,开展新疆地区规模化牛场BVDV基因分型鉴定,对BVDV防控具有重要的指导作用。采集BVDV胶体金抗原检测卡确定为阳性的犊牛抗凝血样品并分离淋巴细胞,在MDBK单层细胞上进行病毒分离,盲传5代,提取培养物RNA,以BVDV5′-UTR、N^_(pro)特异性引物进行逆转录-聚合酶链反应(RT-PCR)检测分析。结果显示,获得BL分离株1株,属于BVDV-1m亚型,研究结果丰富了新疆南疆地区规模牛场BVDV分子流行病学数据库。Many large-scale cattle farms in Xinjiang are infected with bovine viral diarrhea virus(BVDV),and the genotypes are diverse,which brings some difficulties to the prevention and control of the disease,vaccine selection and purification.The genotyping of large-scale cattle farms in Xinjiang plays an important guiding role in the prevention and control of BVDV.In this test,calf anticoagulant samples,confirmed positive for BVDV colloidal gold antigen detection card,were collected and lymphocytes were isolated.Virus isolation was performed on MDBK monolayer cells,blind transmission for five generations,and trophic RNA was extracted for reverse transcript polymerase chain reaction(RT-PCR)analysis with BVDV 5′-UTR and N^_(pro)-specific primers.The results showed that one BL isolate,belonging to the BVDV-1m subtype,and this study enriched the molecular epidemiology database of BVDV in large-scale cattle farms in southern Xinjiang.

关 键 词：牛病毒性腹泻病毒 基因型鉴定 逆转录-聚合酶链反应 

分 类 号：S852.659.6[农业科学—基础兽医学] S858.23[农业科学—兽医学]