鸭肠炎病毒和A型产气荚膜梭菌共感染绿壳蛋鸭十二指肠代谢组学分析  

Duodenal Metabolomic Analysis of DEV and CpA Co-infected Green-shelled Ducks

作　　者：张晨旭 田琴 毕文文 王开功[1,3] 周碧君 文明[1,3] ZHANG Chen-xu;TIAN Qin;BI Wen-wen;WANG Kai-gong;ZHOU Bi-jun;WEN Ming(College of Animal Science,Guizhou University,Guiyang,Guizhou,550025,China;Guizhou Agricultural Vocational College,Qingzhen,Guizhou,551400,China;Guizhou Animal Biological Products Engineering Technology Research Center,Guiyang,Guizhou,550025,China)

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州农业职业学院畜牧水产系,贵州清镇551400 [3]贵州省动物生物制品工程技术研究中心,贵州贵阳550025

出　　处：《动物医学进展》2024年第7期24-30,共7页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(315060703);贵州省百层次创新型人才项目(黔科合平台人才[2016]6009号);贵州省科技平台项目(黔科合平台人才[2018]5253号)。

摘　　要：旨在探究鸭肠炎病毒(DEV)和A型产气荚膜梭菌(CpA)共感染绿壳蛋鸭114 h时十二指肠的差异代谢物变化。试验选取12只健康绿壳蛋鸭,随机分为对照组(CK)和共感染组(TCD)。通过临床观察、剖检病理观察以及PCR检测确定DEV和CpA共感染成功,再使用液相色谱-质谱联用(LC-MS)检测CK组和TCD组感染114 h时鸭十二指肠的代谢物变化,筛选潜在差异代谢物及代谢通路。结果表明,与CK组相比,TCD组鸭十二指肠感染114 h时的总差异代谢物29种,主要有5′-s-甲基-5′-硫腺苷、胞苷、脱氧皮质酮21-葡萄糖苷、腐胺和2-氧吲哚、腐胺、柚皮素、2-氧吲哚、吲哚-3-乙醛等;差异代谢物通过KEGG通路富集分析,共显著富集到22条代谢通路,主要有烟酸酯和烟酰胺代谢、半胱氨酸和蛋氨酸的代谢、酪氨酸代谢、色氨酸代谢、甘油磷脂代谢等,其中与机体免疫和炎症相关的有腐胺、柚皮素、2-氧吲哚、吲哚-3-乙醛、5′-s-甲基-5′-硫腺苷、烟酸脂和半胱氨酸-蛋氨酸代谢、色氨酸代谢和烟酰胺代谢等。综上所述,DEV和CpA共感染绿壳蛋鸭后,可能通过影响腐胺和柚皮素等代谢物以及色氨酸代谢和半胱氨酸-蛋氨酸代谢通路来影响绿壳蛋鸭十二指肠的炎症,为阐明DEV和CpA共感染机制提供科学依据。The aim of this experiment was to investigate the changes of differential metabolites in the duodenum at 114 h of co-infection of green-shelled egg-laying ducks with duck enteritis virus(DEV)and Clostridium perfringens type A(CpA).Twelve healthy green-shelled egg ducks were selected for the experiment and randomly divided into the control group(CK)and the co-infected group(TCD).We determined the success of DEV and CpA co-infection through clinical observation,pathologic observation by dissection and PCR,and then examined the metabolite changes in the duodenum of ducks in the CK group and TCD groups at 114 h of infection by liquid chromatography-mass spectrometry(LC-MS),in order to screen for the potential differential metabolites and metabolic pathways.The results showed that 29 different metabolites were found in the duodenum of ducks in the TCD group at 114 h of infection compared with those in the CK group,including 5′-s-methyl-5′-thioadenosine,cytidine,deoxycorticosterone 21-glucoside,putrescine and 2-oxoindole,putrescine,naringenin,2-oxoindole,and indole-3-glycolide,etc.The metabolic pathways,mainly nicotinic acid,niacin,niacin,2-oxoindole,and indole-3-glycolide,were significantly enriched into 22 metabolic pathways via KEGG enrichment.Metabolic pathways,mainly nicotinic acid ester and nicotinamide metabolism,cysteine and methionine metabolism,tyrosine metabolism,tryptophan metabolism,glycerophospholipid metabolism,etc.Among them,those related to the body's immunity and inflammation were putrescine,naringenin,2-oxoindole,indole-3-glycolaldehyde,5′-s-methyl-5′-thioadenosine,nicotinic acid ester,cysteine Gmethionine metabolism,tryptophan metabolism and nicotinamide metabolism.In summary,DEV and CpA co-infection in green-shelled ducks may affect duodenal inflammation in green-shelled ducks by influencing metabolites such as putrescine and naringenin,as well as tryptophan metabolism and cysteine-methionine metabolism pathways,which provides a scientific basis for elucidating the mechanism of co-infection

关 键 词：鸭肠炎病毒 A型产气荚膜梭菌 代谢组学 十二指肠 

分 类 号：S852.33[农业科学—基础兽医学] S858.32[农业科学—兽医学]