广西地区腹泻仔猪呼肠孤病毒的感染及序列分析  

Infection and Sequence Analysis of Reovirus in Piglets with Diarrhea in Guangxi

作　　者：隆美金 陆颖 李茂宁 易春华 廖梦娟 余科辰 覃一峰[1,2,3] 陈樱 韦祖樟[1,2,3] 黄伟坚 欧阳康[1,2,3] LONG Mei-jin;LU Ying;LI Mao-ning;YI Chun-hua;LIAO Meng-juan;YU Ke-chen;QIN Yi-feng;CHEN Ying;WEI Zu-zhang;HUANG Wei-jian;OUYANG Kang(Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease,College of Animal Science and Technology,Guangxi University,Nanning,Guangxi,530005,China;Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics,Nanning,Guangxi,530005,China;Guangxi Key Laboratory of Animal Reproduction,Breeding and Disease Control,Nanning,Guangxi,530005,China;Guilin Center for Animal Disease Control and Prevention,Guilin,Guangxi,541001,China)

机构地区：[1]广西大学动物科学技术学院/广西高校动物疫病预防与控制重点实验室,广西南宁530005 [2]广西壮族自治区兽用生物制品工程研究中心,广西南宁530005 [3]广西畜禽繁育与疾病防控重点实验室,广西南宁530005 [4]广西桂林市动物疫病预防控制中心,广西桂林541001

出　　处：《动物医学进展》2024年第7期31-37,共7页Progress In Veterinary Medicine

基　　金：广西重点研发计划项目(桂科AB23026082);广西自然科学基金项目(2021GXNSFAA196067);广西大学巴马产教融合研究院专项项目(巴人科20220015);广西百人计划项目

摘　　要：为了解广西地区腹泻仔猪中哺乳动物呼肠孤病毒(MRV)的感染情况及主要流行毒株,应用套式RT-PCR对广西部分地区2020-2022年173份腹泻猪样品进行检测,并选取部分阳性样品进行S 1基因扩增与分析,进一步了解广西地区MRV主要流行毒株的基因特征。结果显示,2020-2022年173份样品MR总阳性率为18%(32/173),各年阳性率分别为29.6%、9.9%和33%。获得1株S 1基因全长序列,序列比对结果发现该序列与其他MRV S 1基因序列的核苷酸同源性为43.4%~97.9%,氨基酸同源性为26.1%~97.8%;遗传进化分析显示,获得的毒株序列为MRV3型,与其他猪源MRV3型毒株ZJ2013、IND/MZ/3013789/reo在同一分支上,同属于谱系Ⅳ。结果表明广西地区猪群存在一定程度的MRV感染,为进一步防控广西腹泻猪群中MRV的流行提供科学依据。In order to understand the infection of mammalian Reovirus(MRV)in diarrhea piglets in Guangxi and major circulating strains,173 samples of diarrhea piglets from 2020 to 2022 were detected by nested RT-PCR.Some of the positive samples were selected to amplify and analyze the S 1 gene,so as to further understand the genetic characteristics of the main epidemic strains of MRV in Guangxi.The results showed that the total positive rate of 173 samples from 2020 to 2022 was 18%(32/173),and the positive rates were 29.6%,9.9%and 33%respectively.In this study,a full-length sequence of S 1 gene was obtained.Sequence alignment showed that the sequence had 43.4%-97.9%nucleotide homology and 26.1%-97.8%amino acid homology with other MRV S 1 gene sequences.Phylogenetic analysis showed that the strain sequence obtained in this study was MRV3,which belonged to lineageⅣin the same branch as other porcine MRV3 strains ZJ2013 and IND/MZ/3013789/reo.The results of this study indicate that there is a certain degree of MRV infection in pigs in Guangxi,which provides a scientific basis for further prevention and control of MRV epidemic in pigs with diarrhea in Guangxi.

关 键 词：哺乳动物呼肠孤病毒 S 1基因 序列分析 感染情况 

分 类 号：S852.652[农业科学—基础兽医学]