全自动酶标免疫分析仪的技术原理与故障案例分析  

Technical principle and cases analysis of faults of fully automatic ELISA analyzer

作　　者：徐炜新 陶苗苗 蔡波 张惠琴 孙杰 Xu Weixin;Tao Miaomiao;Cai Bo;Zhang Huiqin;Sun Jie(Laboratory Department,Jiading District Central Hospital Affiliated to Shanghai University of Medicine&Health Sciences,Shanghai 201800,China)

机构地区：[1]上海健康医学院附属嘉定区中心医院检验科,嘉定201800

出　　处：《中国医学装备》2024年第5期197-201,共5页China Medical Equipment

基　　金：上海市嘉定区自然科学研究课题(JDKW-2022-0014);上海市嘉定区中心医院“双馨培养计划”(SX202206)。

摘　　要：分析全自动酶标免疫分析仪的技术原理及典型故障案例,以提升全自动酶标免疫分析仪的使用效果。通过微粒子酶免疫分析(MEIA)技术完成酶免试验,以主定标、标准定标、定性定标及定标液修正为全自动酶标免疫分析仪的定标方式,提升酶免试验分析精度。通过分析全自动酶标免疫分析仪使用中遇到的洗板机、开机初始化报警、启动软盘、加样臂和滤光器等典型故障案例,提出故障解决应对措施,为全自动酶标免疫分析仪后期维修与管理提供参考。To study the technical principles and case analysis of typical fault of fully automatic enzyme-linked immunosorbent assay(ELISA) analyzer, in order to improve the usage effect of the analyzer. The microparticle enzyme immunoassay(MEIA) analysis technique was used to complete the ELISA test. The main calibration, standard calibration, qualitative calibration and calibration solution correction were used as the calibration methods of the ELISA analyzer to improve the analytic precision of ELISA. By analyzing the cases of typical faults encountered during the use of the fully automatic ELISA analyzer, such as washing machine, startup initialization alarm, boot disk,sampling arm and filter, the corresponding solutions of fault were proposed to provide reference for the maintenance and management of the fully automatic ELISA analyzer at later stage.

关 键 词：全自动酶标免疫分析仪 技术原理 故障 微粒子酶免疫分析(MEIA)技术 荧光物强度 

分 类 号：R197.39[医药卫生—卫生事业管理]