多参数功能MRI评价冷缺血再灌注肾损伤大鼠肾脏血流及氧合水平的价值  

The value of multi-parameter fMRI in evaluating renal blood flow and oxygenation levels in rats with cold ischemia-reperfusion injury

作　　者：徐基磐 陈丽华[2] 任燕 袁仪忠 谢双双[2] 王振[3] 胡占东[4] 沈文[2] XU Jipan;CHEN Lihuan;REN Yan;YUAN Yizhong;XIE Shuangshuang;WANG Zhen;HU Zhandong;SHEN Wen(First Central Clinical College of Tianjin Medical University,Tianjin 300190,China;Department of Radiology,Tianjin First Central Hospital;Organ Transplantation Center of Tianjin First Central Hospital;Department of Pathology,Tianjin First Central Hospital)

机构地区：[1]天津医科大学一中心临床学院,天津300190 [2]天津市第一中心医院放射科 [3]天津市第一中心医院器官移植中心 [4]天津市第一中心医院病理科

出　　处：《国际医学放射学杂志》2024年第3期342-348,共7页International Journal of Medical Radiology

基　　金：国家自然科学基金(81873888,82271971);天津市科技计划项目(21JCYBJC01640)。

摘　　要：目的探讨动脉自旋标记(ASL)和血氧水平依赖(BOLD)技术评估冷缺血再灌注损伤(CIRI)大鼠肾脏血流和血氧微观改变的价值。方法将100只健康雄性SD大鼠随机分为4组,分别为肾脏冷缺血1 h组(CIRI 1 h)、冷缺血2 h组(CIRI 2 h)、冷缺血4 h组(CIRI 4 h)组及假手术组(CIRI 0 h),每组25只。每组随机选取5只分别在术前、术后(1 h、1 d、2 d、5 d)进行ASL及BOLD MRI,测量肾皮质的血流量(RBFCo)值及T_(2)^(*)值(T_(2)^(*)Co)、肾外的髓外带T_(2)^(*)值(T_(2)^(*)OSOM)和肾外髓内带T^(2*)值(T^(2*)ISOM)。随后切除大鼠左肾进行病理学分析并对肾小管损伤程度评分。采用单因素方差分析比较各组间及组内的不同时间点之间MRI参数和大鼠肾小管损伤程度评分之间的差异;采用Pearson相关分析MRI参数间及MRI参数与肾小管损伤程度评分的相关性。结果术后1 h,冷缺血组大鼠肾脏RBFCo、T_(2)^(*)_(Co)、T_(2)^(*)_(OSOM)值均较术前减低(均P<0.05);术后5 d,CIRI 0 h、CIRI 1 h组的RBFCo、T_(2)^(*)_(Co)、T_(2)^(*)_(OSOM)、T^(2*)_(ISOM)值恢复至其术前水平(均P>0.05),CIRI 4 h组上述指标仍低于术前水平(均P<0.05)。术后1 h、1 d、2 d,CIRI 4 h组T_(2)^(*)OSOM值均较其他各组低;术后5 d,CIRI 4 h组RBFCo、T^(2*)_(Co)、T^(2*)_(OSOM)、T^(2*)_(ISOM)值均低于CIRI 0 h组(均P<0.05)。CIRI 2 h组、CIRI 4 h组的术后各时间点的肾小管损伤程度评分均高于其术前(均P<0.05),术后随时间延长,评分呈减低趋势;术后各时间点,CIRI 4 h组评分均较CIRI 0 h、CIRI 1 h组高;术后1 h、2 d、5 d,CIRI 2 h组的评分均较CIRI 0 h、CIRI 1 h组的要高(均P<0.05)。RBF_(Co)、T_(2)^(*)Co、T_(2)^(*)_(OSOM)、T^(2*)_(ISOM)值与肾小管损伤评分均呈中度负相关(r=-0.714、-0.689、-0.518、-0.579,均P<0.001);RBFCo值与T_(2)^(*)_(Co)、T^(2*)_(OSOM)、T^(2*)_(ISOM)值呈中度正相关(r=0.704、0.525、0.612,均P<0.001);T_(2)^(*)_(Co)值与T_(2)^(*)_(OSOM)、T_(2)^(*)_(ISOM)值呈中度正相关(r=0.68Objective To explore the value of arterial spin labeling(ASL)and blood oxygen level dependent(BOLD)techniques in assessing microscopic changes in renal blood flow and blood oxygenation in rats with cold ischemia-reperfusion injury(CIRI)of the kidney.Methods A total of 100 healthy male SD rats were randomly divided into 4 groups:cold ischemia 1-hour group(CIRI 1 h),cold ischemia 2-hour group(CIRI 2 h),cold ischemia 4-hour group(CIRI 4 h),and sham operation group(CIRI 0 h),with 25 rats in each group.Five rats from each group were randomly selected at pre-operation and at 1 hour,1 day,2 days,and 5 days post-operation to undergo ASL and BOLD MRI.The renal cortical blood flow(RBFCo),renal cortical T2*values(T^(2*)_(Co)),outer stripe of outer medulla T2 values(T^(2*)_(OSOM)),and inner stripe of outer medulla T2 values(T^(2*)_(ISOM))values were measured.The left kidney of the rats was then removed for pathological analysis and tubular injury scoring.One-way ANOVA was used to analyze the differences in MRI parameters and tubular injury scores among different groups at various time points and within groups at different time points.Pearson correlation analysis was conducted to determine the correlations between MRI parameters and between MRI parameters and tubular injury scores.Result At 1 hour post-operation,the RBFCo,T^(2*)_(Co),and T^(2*)_(OSOM) values in the cold ischemia group were significantly lower than pre-operation values(all P<0.05).By 5 days post-operation,the RBFCo,T^(2*)_(Co),T^(2*)_(OSOM),and T^(2*)_(ISOM) values in the CIRI 0 h and CIRI 1 h groups had returned to the preoperative level(all P>0.05),whereas these values in the CIRI 4 h group remained lower than pre-operation levels(all P<0.05).At 1 hour,1 day,and 2 days post-operation,the T^(2*)_(OSOM) values in the CIRI 4 h group were lower than those in the other groups(all P<0.05).At 5 days post-operation,the RBFCo,T^(2*)_(Co),T^(2*)_(OSOM) and T^(2*)_(ISOM) values in the CIRI 4 h group were lower than those in the CIRI 0 h group(all P<0.05).The tubular in

关 键 词：肾脏 冷缺血再灌注损伤 动脉自旋标记 血氧浓度依赖 磁共振成像 动物实验 

分 类 号：R445.2[医药卫生—影像医学与核医学]