单细胞多组学测序技术在缺血性脑卒中研究中的应用  

Application of single-cell multi-omics sequencing technology in the study of ischemic stroke

作　　者：孟倩 王依玟 崔娜 白敏 杨乐 丁一 MENG Qian;WANG Yiwen;CUI Na;BAI Min;YANG Le;DING Yi(College of Life Sciences,Northwest University,Xi'an 710069,Shaanxi,China;Department of Pharmacy,Xijing Hospital,Air Force Medical University,Xi'an 710032,Shaanxi,China;Department of Pharmacy,Tangdu Hospital,Air Force Medical University,Xi'an 710038,Shaanxi,China)

机构地区：[1]西北大学生命科学学院,陕西西安710069 [2]空军军医大学西京医院药剂科,陕西西安710032 [3]空军军医大学唐都医院药剂科,陕西西安710038

出　　处：《中国临床药理学与治疗学》2024年第6期690-699,共10页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金(82274313)。

摘　　要：缺血性脑卒中是一种致残率和死亡率较高的急性脑血管疾病,是中国最常见的死亡原因。尽管已有多年研究,但是仍没有脑卒中生物标志物,其分子机制在很大程度上仍不清楚。近十年来,单细胞测序技术作为高速发展起来的一门新兴技术,能够在单个细胞水平上对基因组、转录组、表观遗传组、蛋白组等多组学联合进行高通量测序,为发现生物标志物及解析病理机制提供了新途径。本文详细介绍了单细胞多组学测序技术的进展及其在发现缺血性脑卒中的生物标志物及病理机制等方面的应用,以期为缺血性脑卒中的精准医疗提供有价值的参考。Ischemic stroke is an acute cerebro-vascular disease with high disability and mortality,is the most common cause of death in China.De-spite years of research,there are still no biomark-ers for stroke,and the molecular mechanisms re-main largely unknown.In the past decade,single-cell sequencing technology,as a rapidly developing emerging technology,can conduct high-throughput sequencing of multiple omics including genome,transcriptome,epigenome and proteome at the level of a single cell,providing a new way to discov-er biomarkers and analyze pathological mecha-nisms.In this paper,the progress of single-cell multi-omics sequencing technology and its applica-tion in the discovery of biomarkers,pathological mechanisms and drug development of ischemic stroke are introduced in detail,in order to provide valuable reference for precision medicine of isch-emic stroke.

关 键 词：缺血性脑卒中 单细胞测序 单细胞多组学 生物标志物 精准医疗 

分 类 号：R96[医药卫生—药理学]