针刺百会、神庭对PSCI模型大鼠海马小胶质细胞激活分化的影响研究  

Effects of acupuncture at Baihui and Shenting on the activation and differentiation of hippocampal microglia in PSCI model rats

作　　者：边静 宣立娜 王翠翠 董理[2] 刘春旭 秦晓晔[2] BIAN Jing;XUAN Lina;WANG Cuicui;DONG Li;LIU Chunxu;QIN Xiaoye(Third Affiliated Hospital of Changchun University of Chinese Medicine,Changchun 130117,China;Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学附属第三临床医院,长春130117 [2]长春中医药大学,长春130117

出　　处：《吉林中医药》2024年第5期571-575,共5页Jilin Journal of Chinese Medicine

基　　金：吉林省科技发展计划项目(20210101214JC);长春中医药大学教育基金会项目(11054);吉林省教育厅科学技术研究项目(JJKH20241067KJ)。

摘　　要：目的通过对卒中后认知障碍(PSCI)模型大鼠进行针刺治疗,从海马小胶质细胞激活分化角度探究针刺疗法对PSCI的改善作用及其可能作用机制。方法40只雄性SD大鼠,取8只为假手术组,其余大鼠进行造模,同时满足Zea-Longa评分和水迷宫评分视为PSCI模型大鼠建立成功。造模成功后随机分为模型组、针刺组。针刺组大鼠行百会、神庭针刺治疗,每次15 min,每日1次,连续6 d,休息1 d,4个疗程。假手术组及模型组大鼠仅抓取后放回笼中。采用Zea-Longa评分和水迷宫实验共同评估针刺对模型大鼠学习记忆能力的影响;HE染色评估海马结构及神经元损伤程度;免疫荧光标注激活状态下小胶质细胞特异性标志物Iba-1,M1型特异性标志物CD197及M2型特异性标志物Arg-1;RT-PCR检测CD197及Arg-1 mRNA表达。结果Zea-Longa神经功能评分结果显示,针刺组大鼠较模型组大鼠神经缺损改善明显(P<0.05);水迷宫结果显示,与模型组比较定位巡航测试中针刺组大鼠的平均潜伏期明显缩短(P<0.05);空间探索测试中,针刺组大鼠穿越目标象限的次数比模型组大鼠多(P<0.05);HE染色显示针刺组较模型组神经元数量增多;免疫荧光及RT-PCR显示,与模型组比较,针刺组小胶质细胞标志物Iba-1及M1型标志物CD197减少,M2型标志物Arg-1增多(P<0.05)。结论针刺百会、神庭可抑制PSCI模型大鼠海马小胶质细胞激活分化,促进小胶质细胞M1表型向M2表型转化,改善认知功能。Objective To explore the improvement effect of acupuncture therapy on post-stroke cognitive impairment(PSCI)in rats from the perspective of activation and differentiation of hippocampal microglia,as well as its possible mechanisms.Methods Forty male SD rats were used,with 8 rats in the sham operation group and the remaining undergoing modeling.Successful modeling was confirmed by meeting both the Zea-Longa scores and the water maze scores for PSCI in rats.After successful modeling,the rats were randomly divided into a model group and an acupuncture group.The rats in the acupuncture group received an acupuncture treatment at Baihui and Shenting points for 15 minutes per session,once a day for 6 consecutive days with a rest day in between,for a total of 4 courses,while the rats in the sham operation group and the model group were only dealt with handling.The effects of acupuncture on the learning and memory abilities of the model rats were evaluated using the Zea-Longa scores and the water maze experiment.HE staining was used to assess the hippocampal structure and the degree of neuronal damage,while immunofluorescence labeling was used to detect the activation state of specific markers for microglia(Iba-1),M1-type microglia(CD197),and M2-type microglia(Arg-1).RT-PCR was used to detect the mRNA expression of CD197 and Arg-1.Results The Zea-Longa neurological function scores showed that the neurological deficits in the acupuncture group were improved compared to those in the model group rats(P<0.05).In the water maze test,the acupuncture group had a significantly shorter average latency period in the spatial probe test compared to that in the model group(P<0.05).In the spatial exploration test,the acupuncture group crossed the target quadrant for more times than the model group(P<0.05).HE staining showed an increase in the number of neurons in the acupuncture group compared to that in the model group.Immunofluorescence and RT-PCR analysis revealed that in the acupuncture group,the expression of the microglial marker

关 键 词：针刺 卒中后认知障碍 小胶质细胞 

分 类 号：R245.3[医药卫生—针灸推拿学]