六君子汤中理气药陈皮挥发性成分与胃肠动力调节关联性研究  

Study on the correlation between dried tangerine peel volatile components in Liujunzi decoction and regulation of gastrointestinal motility

作　　者：吕邵娃[1] 郭红丽 李影[1] 孙爽[1] LV Shaowa;GUO Hongli;LI Ying;SUN Shuang(Key Laboratory of Basic and Application Research of Beiyao,Ministry of Education,Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学教育部北药基础与应用研究重点实验室,哈尔滨150040

出　　处：《吉林中医药》2024年第5期582-589,共8页Jilin Journal of Chinese Medicine

基　　金：黑龙江省博士后科研启动金项目(LBH-Q21189);国家自然科学基金青年科学基金项目(81803985);黑龙江省“头雁”团队支持项目(黑龙江省头雁行动领导小组文件〔2019〕5号)。

摘　　要：目的从胃动力调节角度分析理气药陈皮挥发性成分在补气方剂六君子汤中的理气作用,比较不同陈化年份的陈皮挥发性成分对脾虚大鼠胃排空和小肠推进的影响,进而挖掘六君子汤中理气药陈皮挥发性成分与调节脾虚大鼠胃肠动力的关联性成分。方法采用GC构建15批不同陈化年份的陈皮药材挥发油类成分的指纹图谱,以胃排空和小肠推进为指标评价陈皮挥发性成分改善胃肠动力作用,采用偏最小二乘回归分析以及灰度分析法分析指纹图谱与调节脾虚大鼠胃肠动力相关性。结果陈皮挥发油成分均可促进胃排空及小肠推进,其在六君子汤中的增益作用强度以5、10年最佳。在15批不同陈化年份的陈皮药材挥发油类成分的GC图谱中,峰3、峰4、峰9为发挥胃肠道调节作用的可能药效成分。结论六君子汤中理气药陈皮挥发性成分通过增益脾虚大鼠胃动力达到理气补气之效,此增益作用为多种有效成分共同起效的结果。该研究初步揭示了六君子汤中理气药陈皮挥发性成分的理气作用与脾虚胃动力调节的关联性,为探索补气方剂六君子汤中陈皮理气作用的物质基础提供一种新思路。Objective From the perspective of regulating gastric motility,to analyze the qi-regulating effect of volatile components in dried tangerine peel,a qi-regulating drug,in the qi-tonifying formula Liujunzi decoction,to compare the effects of different-aged dried tangerine peel volatile components on gastric emptying and small intestine propulsion in rats with spleen deficiency,and then to explore the association between dried tangerine peel volatile oil and gastrointestinal motility regulation in rats with spleen deficiency.Methods The finger prints of volatile oil in 15 batches of dried tangerine peel from different origins and years were established by GC.The effect of volatile components in dried tangerine peel on improving gastrointestinal motility was evaluated by using gastric emptying and small intestine propulsion as indicators.Finally,partial least square regression analysis and gray scale analysis were used to analyze the correlation between their finger prints and the regulation of gastrointestinal motility in rats with spleen deficiency.Results The volatile components of 15 batches of dried tangerine peel could promote gastric emptying and small intestine propulsion,and their gain intensity in Liujunzi decoction was best for those with 5 or 10 years.In the GC chromatograms of volatile components of 15 batches of different-aging years,the myrcene(peak 3),D-limonene(peak 4),and peak 9 were the potential medicinal components that improved gastrointestinal motility.Conclusion Volatile oil in the qi-regulating dried tangerine peel volatile components in Liujunzi decoction have the synergistic effect of regulating and replenishing qi through the regulation of gastric motility in rats with spleen deficiency,which is the result of multiple active components.This study preliminarily reveals the relationship between the qi-regulating effect of volatile components in dried tangerine peel in Liujunzi decoction and the stomach motility regulation in rats with spleen deficiency,providing a new idea for exploring the m

关 键 词：六君子汤 陈皮挥发油 指纹图谱 胃肠动力 谱效关系 

分 类 号：R284.1[医药卫生—中药学]