高效液相色谱法测定益肾排石颗粒中黄芩苷含量  

Determination of baicalin in Yishen Paishi granules by HPLC

作　　者：马永璠 薄天儒 于亚杰 刘艳华[1] 初洪波[1] MA Yongfan;BO Tianru;YU Yajie;LIU Yanhua;CHU Hongbo(Changchun University of Chinese Medicine,Changchun 130117,China;Jilin Guowen Hospital,Siping 136100,China;Siping Product Quality Inspection Institute,Siping 136000,China)

机构地区：[1]长春中医药大学,长春130117 [2]吉林国文医院,吉林四平136100 [3]四平市产品质量检验院,吉林四平136000

出　　处：《吉林中医药》2024年第5期590-594,共5页Jilin Journal of Chinese Medicine

基　　金：吉林省科技发展计划项目(20200404068YY);吉林省自然科学基金项目(YDZJ202201ZYTS237)。

摘　　要：目的建立益肾排石颗粒中黄芩苷含量的HPLC测定方法,为益肾排石颗粒的质量控制提供科学、可靠依据。方法采用高效液相色谱法,岛津ODS-2C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相为0.2%磷酸水(A)-甲醇(B)梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长280 nm,进样量均为10μL。结果该方法黄芩苷峰面积和浓度之间线性关系良好,r=1.0000;精密度、稳定性均符合要求;平均加样回收率为103.29%,RSD为2.38%;三批样品中黄芩苷的平均含量为6.12 mg/g,RSD值为1.71%。结论本研究方法重复性好,准确度高,可用于复方益肾排石颗粒中黄芩苷的含量测定。Objective To establish an HPLC method for the determination of baicalin in Yishen Paishi granules,and to provide a scientific and reliable basis for the quality control of the medicine.Methods High performance liquid chromatography(HPLC)was performed on Shimadzu ODS-2C_(18)column(250 mm×4.6 mm,5μm),with its mobile phase as a gradient elution of 0.2%phosphoric acid water(A)-methanol(B),with its flow rate at 1.0 mL/min,its column temperature at 30℃,its detection wavelength at 280 nm,and its sample size all at 10μL.Results The linear relationship between the peak area and the concentration of baicalin was good,r=1.0000.Its precision and stability met the requirements.Its average recovery was 103.29%and RSD was 2.38%.The average content of baicalin in the three batches of samples was 6.12 mg/g,and the RSD value was 1.71%.Conclusion The method has a good repeatability and a high accuracy,and can be used for the determination of baicalin in compound Yishen Paishi granules.

关 键 词：益肾排石颗粒 黄芩苷 高效液相色谱法 含量测定 

分 类 号：R286.0[医药卫生—中药学]