参松养心胶囊通过铁死亡途径保护心肌缺血再灌注损伤研究  

Shensong Yangxin Capsule plays a protective role in myocardial ischemia-reperfusion injury through iron death pathway

作　　者：贾凌梅 陈亚丽 贾敏 刘畅[1] 栾莹莹 张颖[1] JIA Lingmei;CHEN Yali;JIA Min;LIU Chang;LUAN Yingying;ZHANG Ying(The Second Hospital of Hebei Medical University,Shijiazhuang 050000,Hebei,China)

机构地区：[1]河北医科大学第二医院,河北石家庄050000

出　　处：《现代中西医结合杂志》2024年第8期1072-1076,共5页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：河北省卫生健康委科研基金项目(20221062)。

摘　　要：目的探究参松养心胶囊对心肌缺血再灌注损伤的保护作用及其机制。方法实验设对照组、糖氧剥夺/复氧组、糖氧剥夺/复氧+参松养心低剂量组、糖氧剥夺/复氧+参松养心中剂量组、糖氧剥夺/复氧+参松养心高剂量组。对照组H9c2细胞常规培养,其余组H9c2细胞均进行糖氧剥夺/复氧造模模拟心肌细胞缺血再灌注损伤,糖氧剥夺/复氧+参松养心低、中、高剂量组在造模前加入浓度为0.25μg/mL、0.5μg/mL、1.0μg/mL的参松养心胶囊悬液培养24 h。采用MTT法检测细胞存活率,试剂盒检测细胞中活性氧(ROS)、丙二醛(MDA)、超氧化物歧化酶(SOD)及Fe^(2+)含量,Western blot法检测细胞中脂质过氧化相关因子[长链酯酰辅酶A合成酶4(ACSL4)、谷胱甘肽过氧化物酶4(GPX4)]及铁死亡途径相关蛋白[转铁蛋白受体1(TfR1)、缺氧诱导因子-1α(HIF-1α)、铁储存蛋白(FTH1)、核受体共激活因子4(NCOA4)、血红素加氧酶-1(HO-1)]的表达情况。结果与对照组比较,糖氧剥夺/复氧组细胞存活率、细胞中SOD含量及细胞中GPX4、FTH1、NCOA4蛋白相对表达量均明显降低(P均<0.05),细胞中ROS、MDA、Fe^(2+)含量及细胞中ACSL4、TfR1、HIF-1α、HO-1蛋白相对表达量均明显升高(P均<0.05)。与糖氧剥夺/复氧组比较,糖氧剥夺/复氧+参松养心各组细胞存活率、细胞中SOD含量及细胞中GPX4、FTH1、NCOA4蛋白相对表达量均明显升高(P均<0.05),细胞中ROS、MDA、Fe^(2+)含量及细胞中ACSL4、TfR1、HIF-1α、HO-1蛋白相对表达量均明显降低(P均<0.05)。结论参松养心胶囊可通过调控铁死亡途径减轻氧化应激反应,保护缺血再灌注损伤心肌细胞。Objective It is to explore the protective effect and its internal mechanism of Shensong Yangxin Capsule on myocardial ischemia-reperfusion injury.Methods H9c2 cells were randomly divided into control group,glyoxy-deprivation/reoxygenation(OGD/R)group,OGD/R+low dose ShensongXinjiang group,OGD/R+medium dose Shensongxinjiang group and OGD/R+high dose Shensongxinjiang group.The H9c2 cells were normal cultured in the control group,and the cells in the other groups were modeled with OGD/R to simulate ischemia-reperfusion injury in cardiomyocytes.The OGD/R+low dose ShensongXinjiang group,OGD/R+medium dose Shensongxinjiang group and OGD/R+high dose Shensongxinjiang group were respectively cultured with 0.25μg/mL,0.5μg/mL,1.0μg/mL Shensong Yangxin capsule solution before modeling.The cell survival rate was detected by MTT assay,the contents of reactive oxygen species(ROS),malondialdehyde(MDA),superoxide dismutase(SOD)and Fe^(2+)in the cells were detected by kits,the expression of lipid peroxidation-related factors[acyl-CoA synthetase long-chain family member 4(ACSL4),glutathione peroxidase 4(GPX4)]and iron-death pathway-related proteins[transferrin receptor 1(TfR1),hypoxia-inducible factor-1α(HIF-1α),ferritin heavy chain 1(FTH1),nuclear receptor coactivator 4(NCOA4),heme oxygenase-1(HO-1)]were detected by Western blot assay.Results Compared with the control group,the survival rate,the content of SOD and relative expressions of GPX4,FTH1,and NCOA4 proteins in the cells of OGD/R group were significantly decreased(all P<0.05),while the contents of ROS,MDA,and Fe^(2+)and relative expressions of ACSL4,TfR1,HIF-1α,and HO-1 proteins in the cells were significantly increased(all P<0.05).Compared with the OGD/R group,the survival rate,the content of SOD and relative expressions of GPX4,FTH1,and NCOA4 proteins in the cells of each OGD/R+Shensongxinjiang group were significantly increased(all P<0.05),while the contents of ROS,MDA,and Fe^(2+)and relative expressions of ACSL4,TfR1,HIF-1α,and HO-1 proteins in the cells were sign

关 键 词：参松养心胶囊 心肌缺血再灌注损伤 铁死亡 氧化应激 

分 类 号：R541[医药卫生—心血管疾病]