丹酚酸B对肝损伤小鼠肠道菌群和短链脂肪酸代谢的影响  

Effect of salvianolic acid B on intestinal flora and short chainfatty acid metabolism in mice with liver injury

作　　者：周喆 胡诗安 胡雨瞳 谭洋[1] 裴刚[1] 李玲[1] ZHOU Zhe;HU Shi-an;HU Yu-tong;TAN Yang;PEI Gang;LI Ling(Key Laboratory of Modern Research of TCM,Education Department of Hunan Province,College of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学药学院湖南省普通高等学校中药现代化重点实验室,长沙410208

出　　处：《天然产物研究与开发》2024年第5期748-754,824,共8页Natural Product Research and Development

基　　金：国家自然科学基金(82174271)。

摘　　要：本研究旨在探讨丹酚酸B(salvianolic acid B,Sal B)对小鼠肝损伤的干预作用,以及对肠道菌群和短链脂肪酸(short-chain fatty acids,SCFAs)代谢的影响。通过皮下注射20%CCl 4溶液建立小鼠肝损伤模型,在造模的同时给予Sal B和阳性药2-脱氧-葡萄糖(2-deoxy-D-glucose)灌胃干预,干预结束后通过苏木素-伊红(hematoxylin-eosin,HE)、Masson、天狼星红染色观察小鼠肝脏病理变化;采用生化试剂盒检测血清谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)水平;运用16S rRNA测序技术检测小鼠肠道菌群结构变化;通过气相色谱法测定小鼠粪便中SCFAs的含量。结果显示,Sal B可以显著降低CCl 4诱导的肝损伤小鼠血清ALT、AST水平(P<0.001),并减轻肝组织病理损伤。测序结果显示,Sal B可以部分恢复肝损伤小鼠的肠道菌群结构,同时显著增加肠道中异丁酸、异戊酸、丙酸和戊酸的含量(P<0.001)。本实验揭示了Sal B可以减轻CCl 4所诱导的小鼠肝损伤,机制可能与其对肠道菌群和SCFAs紊乱的调节有关。The objective of this study was to examine the impact of salvianolic acid B(Sal B)on liver injury in mice,as well as its effects on intestinal flora and short-chain fatty acids(SCFAs).To establish a mouse liver injury model,a subcutaneous injection of 20%CCl 4 solution was administered.At the same time,mice in treatment groups were subjected to Sal B and the positive drug 2-deoxy-D-glucose gavage intervention,respectively.After the administration,the pathological changes of liver tissue were observed by hematoxylin-eosin(HE),Masson,and Sirius red staining,and the levels of serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)were detected by biochemical kits.16S rRNA sequencing technology was used to detect the changes of intestinal microbiota in mice.Additionally,the contents of SCFAs in mice feces were determined through gas chromatography.The results indicate that Sal B has a significant effect on reducing serum ALT and AST levels(P<0.001)and mitigating liver pathological damage.Moreover,Sal B was revealed to partly restore the composition of intestinal flora in mice with liver injury,as evidenced by the sequencing data.Furthermore,the administration of Sal B resulted in a significant increase in the concentrations of isobutyric acid,isovaleric acid,propionate acid,and valeric acid in the feces from mice with liver injury(P<0.001).Consequently,the above results could conclude that Sal B possesses the ability to alleviate liver injury induced by CCl 4 in mice.The mechanism is potentially related to its regulation of the balance of intestinal flora and the restoration of the contents of SCFAs.

关 键 词：丹酚酸B 肝损伤 肠道菌群 短链脂肪酸 

分 类 号：R285.5[医药卫生—中药学]