猫疱疹病毒1型的检测及一株分离毒株的致病性  

Detection of Feline Herpesvirus Type 1 and Pathogenicity of an Isolated Strain

作　　者：邓梏男 张家祺 保志鹏[4] 陈涛云 喻琦胜 丁露 朱晨曦 王怡 任玉鹏 贺超 张斌[1,2] DENG Gunan;ZHANG Jiaqi;BAO Zhipeng;CHEN Taoyun;YU Qisheng;DING Lu;ZHU Chenxi;WANG Yi;REN Yupeng;HE Chao;ZHANG Bin(College of Animal and Veterinary Sciences,Southwest Minzu University,Chengdu 610041,China;Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Ministry of Education/Sichuan Provine,Chengdu 610041,China;Sichuan Provincial Veterinary Drug Supervision Institute,Chengdu 610041,China;Yunnan Vocational and Technical College of Agriculture,Kunming 650212,China)

机构地区：[1]西南民族大学畜牧兽医学院,成都610041 [2]青藏高原动物遗传资源保护与利用教育部/四川省重点实验室,成都610041 [3]四川省兽药监察所,成都610041 [4]云南农业职业技术学院,昆明650212

出　　处：《畜牧兽医学报》2024年第5期2253-2258,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家农业产业技术体系四川兽药创新团队(SCCXTD-2020-18);西南民族大学人才支持基金项目(RQD2021004)。

摘　　要：旨在调查成都地区的猫疱疹病毒1型(feline herpesvirus-1, FHV-1)的流行及遗传变异情况。试验选取2020—2023年从成都地区采集的178份具有呼吸道症状猫的眼、鼻及口咽拭子进行FHV-1核酸检测、gE基因遗传进化分析、FHV-1的分离鉴定和致病性研究。结果发现,成都地区FHV-1阳性率为11.8%(21/178);其中宠物医院FHV-1阳性率为6.4%(7/110),猫舍FHV-1阳性率为20.6%(14/68),表明在猫舍中FHV-1感染率更高。FHV-1 gE基因的遗传进化分析结果表明,本研究扩增出的5株gE基因和疫苗株的核苷酸同源性在99.1%~100%,并发现PX-9株存在3个独特的氨基酸突变位点(L259Q、C294I、W295F)。通过CRFK细胞对5份阳性样本进行病毒的分离培养,结果PX-9毒株被成功分离鉴定。对PX-9毒株进行动物回归试验,结果表明攻毒组猫只表现出眼鼻分泌物增多,打喷嚏等临床症状,在接毒后第4天病毒拷贝数最高,为9.05×10^(7) copies·μL^(-1),且排毒期较长;攻毒猫只FHV-1病毒载量检测结果显示,气管及肺组织病毒拷贝数分别为1.7×10^(7)和3.5×10^(4) copies·μg^(-1),其它组织核酸阴性。综上表明,在成都地区仍然存在FHV-1流行,并且流行毒株有较强的致病性。The aim of this study was to investigate the prevalence and genetic diversity of feline herpesvirus-1(FHV-1)in Chengdu city.From 2020 to 2023,a total of 178 conjunctiva,pharynx and nose swabs were collected from cats with respiratory symptoms in Chengdu city.The collected samples were subjected to FHV-1 PCR detection,gE gene genetic evolutionary analysis,viral isolation and identification,as well as pathogenicity.The results showed that,the FHV-1 positivity rate in Chengdu city was 11.8%(21/178).In which the FHV-1 positivity rate of veterinary hospitals was 6.4%(7/110)and 20.6%(14/68)in catteries,suggested that the higher rate of FHV-1 infection was in catteries.The genetic evolutionary analysis of five FHV-1 gE genes showed that the nucleotide homology was 99.1%~100%to the vaccine strains,and three unique amino acid mutation sites were found in PX-9 strain(L259Q,C294I,W295F).Five positive samples were isolated and cultured by CRFK cell,only PX-9 strain was successfully isolated and identified from CRFK cells.The results of animal regression experiment by PX-9 strain showed that all cats in the infected group exhibited clinical symptoms,such as increased ocular,nasal secretions and sneezing.The highest viral load was found on the 4th day after inoculation(9.05×10^(7) copies·μL-1),and the detoxification period is relatively long.The results of FHV-1 viral load were 1.7×10^(7) and 3.5×10^(4) copies·μg^(-1) in trachea and lungs of infected group,respectively.The other tissues were negative for FHV-1 DNA.The results of this study indicated that FHV-1 was still prevalent in Chengdu and the prevalent strains had highly pathogenicity.

关 键 词：FHV-1 流行情况 遗传变异 动物回归试验 

分 类 号：S852.659.1[农业科学—基础兽医学] S858.293[农业科学—兽医学]