表达漆酶的重组酿酒酵母菌株同步糖化发酵产乙醇特性研究  

SIMULTANEOUS SACCHARIFICATION AND CELLULOSICETHANOL FERMENTATION OF RECOMBINANT YEASTSTRAIN WITH EXPRESSING LACCASE

作　　者：李娟娟 王志鹏 朱士论 叶禹彤 路佳琪 田沈[1] Li Juanjuan;Wang Zhipeng;Zhu Shilun;Ye Yutong;Lu Jiaqi;Tian Shen(College of Life Sciences,Capital Normal University,Beijing 100048,China)

机构地区：[1]首都师范大学生命科学学院,北京100048

出　　处：《太阳能学报》2024年第5期172-177,共6页Acta Energiae Solaris Sinica

基　　金：国家自然科学基金面上项目(31971202);国家科技支撑计划(2019YFB1503802)。

摘　　要：将来源于硬毛拴菌(Trametes trogii CICC 2689)的lcc1基因通过Di-CRISPR技术整合到酿酒酵母Y6中以实现漆酶的稳定表达,考察漆酶在酚类抑制剂及同步糖化发酵中的抗逆作用。结果显示:平板拮抗和液体摇瓶实验中,重组菌株Y6/Lac的生长状况、对数期生长速率及延滞期较对照菌株Y6均表现出相应优势;同时Y6/Lac对碱木质素的降解率提高41%,同步糖化发酵产乙醇效率提高14%。Lcc1 gene from Trametes trogii CICC 2689 was integrated into Saccharomyces cerevisiae Y6 by Di-CRISPR to achieve stable expression of laccase,and to investigate the stress resistance of laccase on phenolic inhibitors and simultaneous saccharification and fermentation.The results showed that the recombinant strains Y6/Lac in the medium containing inhibitors had shorter lag phase,higher exponential phase growth rate and final biomass than Y6.At the same time,the degradation rate of alkali lignin by Y6/Lac increased by 41%,and the production efficiency of ethanol by simultaneous saccharification and fermentation increased by 14%.

关 键 词：重组酿酒酵母 漆酶 Di-CRISPR 同步糖化发酵 纤维素乙醇 

分 类 号：TK6[动力工程及工程热物理—生物能]