苗药了哥王中瑞香烷二萜原酸酯类成分及其抗肿瘤活性研究  

Daphnane Diterpenoid Orthoesters of Miao Medicine Wikstroemia indica and the Antitumor Activities

作　　者：国光梅 谭琳 苑春茂 穆淑珍 汪冶[3] GUO Guang-mei;TAN Lin;YUAN Chun-mao;MU Shu-zhen;WANG Ye(Natural Products Research Center of Guizhou Province,Guiyang 550002,China;The Affiliated Hospital of Guizhou Medical Uni-versity,Guiyang 550002,China;Lishui University,Lishui 323000,China)

机构地区：[1]贵州省天然产物研究中心,贵州贵阳550002 [2]贵州医科大学附属医院,贵州贵阳550002 [3]丽水学院,浙江丽水323000

出　　处：《中药材》2024年第1期81-84,共4页Journal of Chinese Medicinal Materials

基　　金：贵州省科技支撑计划项目(黔科合支撑[2021]一般110)。

摘　　要：目的:研究苗药了哥王Wikstroemia indica(L.) C.A.Mey.中瑞香烷二萜原酸酯类成分及其抗肿瘤活性。方法:采用硅胶柱色谱、RP-C_(18)反相柱色谱、Sephadex LH-20葡聚糖凝胶柱色谱和半制备液相色谱等分离纯化技术获得了哥王中的瑞香烷二萜原酸酯类成分,再根据其理化性质和波谱数据鉴定各化合物的结构,并采用MTT法对化合物1、3、5进行抗肝癌HepG_(2)细胞株的活性评价。结果:从苗药了哥王中分离得到5个瑞香烷二萜原酸酯类化合物,分别鉴定为:6α,7α-dihydyoxyhuratoxin(1)、simplexin(2)、12β-acetoxyhuratoxin(3)、excoecafolin D(4)、pimelea factor P_(2)(5)。此类化合物对肝癌HepG_(2)细胞的增殖均有明显的抑制作用。结论:其中,化合物1为新化合物,化合物2~5为首次从该植物中分离得到,化合物3对肝癌HepG_2细胞增殖的抑制作用最佳,IC_(50)值为(5.75±0.15)μg/mL。Objective:To study the daphnane diterpenoid orthoesters of Miao Medicine Wikstroemia indica and the antitumor activities.Methods:The daphnane diterpenoid orthoesters of Wikstroemia indica were isolated and purified by silica gel column chromatography,RP-C18 reversed phase column chromatography,Sephadex LH-20 glucan gel column chromatography and semi-preparative liquid chromatography,etc.The structures were identified by the physicochemical characteristics and spectral data.The cytotoxic activities of compounds 1,3,5 against hepatoma HepG_(2) cells line were evaluated by MTT method.Results:Five daphnane diterpenoid orthoesters were isolated from Miao medicine Wikstroemia indica and identified as 6α,7α-dihydyoxyhuratoxin(1),simplexin(2),12β-acetoxyhuratoxin(3),excoecafolin D(4),pimelea factor P2(5).These compounds showed the significant cytotoxicities against HepG_(2) cells.Conclusion:Among them,compound 1 is a new compound,compounds 2 to 5 are isolated from Wikstroemia indica for the first time.Compound 3 has significant effect on inhibition of HepG_(2) cell proliferation with IC_(50) value of(5.75±0.15)μg/mL.

关 键 词：苗药 了哥王 瑞香烷二萜原酸酯 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学] R285.5