云南重楼果皮中1个新的C_(21)甾体皂苷  

A New C_(21)Steroid Saponin from the Pericarps of Paris polyphylla var.yunnanensis

作　　者：苏会梅 聂伟 赵邦 李倩 冯莉萍 邓亚 万近福 SU Hui-mei;NIE Wei;ZHAO Bang;LI Qian;FENG Li-ping;DENG Ya;WAN Jin-fu(Department of Nuclear Medicine,the 926 Hospital of PLA,Kaiyuan 661699,China;Yunnan Institute of Materia Medical/Key Laboratory for TCM and Ethnic Medicine Innovation and Manufacturing Enterprises in Yunnan Province,Kunming 650111,China;College of Pharmacy,Kunming Medical University/Yunnan Key Laboratory of Pharmacology for Natural Drugs,Kunming 650500,China)

机构地区：[1]中国人民解放军第926医院核医学科,云南开远661699 [2]云南省药物研究所/云南省中药和民族药新药创制企业重点实验室,云南昆明650111 [3]昆明医科大学药学院/云南省天然药物药理重点实验室,云南昆明650500

出　　处：《中药材》2024年第1期85-90,共6页Journal of Chinese Medicinal Materials

摘　　要：目的:研究云南重楼果皮中的甾体皂苷类成分。方法:采用HPD-100大孔吸附树脂柱、正相硅胶柱、ODS柱、Sephadex LH-20葡聚糖凝胶柱和半制备型高效液相色谱等方法对云南重楼果皮70%乙醇渗漉提取物中的甾体皂苷类成分进行系统研究,通过现代波谱学技术结合参考文献对化合物结构进行鉴定。结果:从云南重楼果皮中分离得到8个甾体皂苷类化合物,分别鉴定为:17α-hydroperoxide pregn-5-en-20-one-3-O-α-L-rhamnopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside(1)、重楼皂苷Ⅳ(2)、ypsilandroside L(3)、重楼皂苷Ⅲ(4)、pregna-5,16-dien-3β-ol-20-one-3β-O-α-L-rhamnopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside(5)、pregna-5,16-dien-3β-ol-20-one-3β-O-α-L-rhamnopyranosyl-(1→2)-[α-L-arabinopyranosyl-(1→4)]-β-D-glucopyranoside(6)、pregna-5,16-dien-3β-ol-20-one-3β-O-α-L-rhamnopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside(7)、aethioside A(8)。结论:其中,化合物1为一个新的C_(21)甾体皂苷类化合物,化合物3、5、6、8为首次从重楼属植物中分离得到。Objective:To study the chemical constituents of steroid saponin from the pericarps of Paris polyphylla var.yunnanensis.Methods:The chemical constituents of steroid saponin from the 70%ethanol-percolated extract of the pericarps of Paris polyphylla var.yunnanensis were systematically studied by using HPD-100 macroporous resin,forward silica gel column chromatography,reverse ODS column chromatography,Sephadex LH-20 and semi-preparative high performance liquid chromatography.The structures of the isolated compounds were identified by the spectroscopic data with references.Results:Eight steroid saponin were isolated from the pericarps of Paris polyphylla var.yunnanensis and identified as 17α-hydroperoxide pregn-5-en-20-one-3-O-α-L-rhamnopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside(1),polyphyllosideⅣ(2),ypsilandroside L(3),polyphyllosideⅢ(4),pregna-5,16-dien-3β-ol-20-one-3β-O-α-L-rhamnopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside(5),pregna-5,16-dien-3β-ol-20-one-3β-O-α-L-rhamnopyranosyl-(1→2)-[α-L-arabinopyranosyl-(1→4)]-β-D-glucopyranoside(6),pregna-5,16-dien-3β-ol-20-one-3β-O-α-L-rhamnopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside(7),aethioside A(8),respectively.Conclusion:Among them,compound 1 is a new C_(21)steroid saponin,compounds 3,5,6,8 are isolated from Paris genus for the first time.

关 键 词：云南重楼果皮 C_(21)甾体皂苷 化学成分 结构鉴定 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]