密花美登木化学成分及抗肿瘤活性研究  

Study on the Chemical Constituents and Antitumor Activity of Maytenus confertiflora

作　　者：贾学恭 黄园园 张雯艳[1] 梁旭 邓富声 邓巧玉 袁经权[1] JIA Xue-gong;HUANG Yuan-yuan;ZHANG Wen-yan;LIANG Xu;DENG Fu-sheng;DENG Qiao-yu;YUAN Jing-quan(Scientific Experiment Center,Guangxi University of Traditional Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学科学实验中心,广西南宁530200

出　　处：《中药材》2024年第1期96-101,共6页Journal of Chinese Medicinal Materials

基　　金：广西科技创新能力与条件建设(桂科能10100027-3);广西中医药大学博士科研启动基金资助项目(2018BS048)。

摘　　要：目的:研究卫矛科美登木属植物密花美登木Maytenus confertiflora J.Y.Luo et X.X.Chen茎叶的化学成分及其体外抗肿瘤活性。方法:采用正相硅胶、Sephadex LH-20葡聚糖凝胶、MCI以及Semi-Prep HPLC对密花美登木醇提物进行分离纯化,并采用MTT法对三萜化合物进行体外抗肿瘤活性评价。结果:从密花美登木中分离得到17个化合物,分别鉴定为:tridec-1-ene(1)、bis(2-ethylhexyl)phthalate(2)、二十五烷(3)、β-amyrenone(4)、3-hydroxyfriedel-3-en-2-one(5)、β-谷甾醇(6)、28,29-dihydroxyfriedelan-3-one(7)、friedelin(8)、3α-hydroxyfriedel-2-one(9)、3-oxofriedelan-28-oic acid(10)、canophyllol(11)、3-oxo-29-hydroxyfiedelane(12)、pentadecanoic acid(13)、华中冬青素(14)、lingueresinol(15)、飞龙掌血内酯(16)、橙黄胡椒酰胺乙酸酯(17)。结论:所有化合物均为首次从该植物中分离得到。化合物7对Eca-109、EJ细胞具有较强抑制作用,其IC_(50)分别为27.67、30.09μmol/L;化合物12对HepG_(2)、PANC-1细胞均具有较好的抑制作用,其IC_(50)分别为49.42、51.87μmol/L。Objective:To study the chemical constituents from the stems and leaves of Maytenus confertiflora and its anti-tumor activities in vitro.Methods:The ethanol extract of Maytenus confertiflora was isolated and purified by using normal phase silica gel,Sephadex LH-20,MCI and semi-preparative HPLC,and the in vitro antitumor activity of the triterpenoids was evaluated by the MTT method.Results:Seventeen compounds were isolated from Maytenus confertiflora and identified as tridec-1-ene(1),bis(2-ethylhexyl)phthalate(2),pentacosane(3),β-amyrenone(4),3-hydroxyfriedel-3-en-2-one(5),β-sitosterol(6),28,29-dihydroxyfriedelan-3-one(7),friedelin(8),3α-hydroxyfriedel-2-one(9),3-oxofriedelan-28-oic acid(10),canophyllol(11),3-oxo-29-hydroxyfiedelane(12),pentadecanoic acid(13),icariol A2(14),lingueresinol(15),toddalolactone(16),aurantiamide acetate(17),respectively.Conclusion:All compounds are isolated from Maytenus confertiflora for the first time.Compound 7 has a strong inhibitory effect on Eca-109 and EJ cells with IC_(50) of 27.67 and 30.09μmol/L,respectively.Compound 12 has a good inhibitory effect on HepG2 and PANC-1 cells,with IC_(50) of 49.42 and 51.87μmol/L,respectively.

关 键 词：密花美登木 化学成分 三萜 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学] R285.5