薄层色谱法分离菠菜色素实验的绿色改进  

Green Improvement of Thin Layer Chromatography for Separation of Spinach Pigments

作　　者：雷霓[1] 孙耀冉[1] 贾晓亚 刘宏飞 乔哲佳 LEI Ni;SUN Yao-ran;JIA Xiao-ya;LIU Hong-fei;QIAO Zhe-jia(School of Chemical Engineering,Shijiazhuang University,Shijiazhaung,Hebei 050035,China)

机构地区：[1]石家庄学院化工学院,河北石家庄050035

出　　处：《石家庄学院学报》2024年第3期41-47,共7页Journal of Shijiazhuang University

摘　　要：薄层色谱法(TLC)是一种快速分离和定性分析少量物质的常用实验技术,在原有薄层色谱法分离菠菜色素的实验中,所选择的展开剂中使用大量一类致癌物苯,实验过程中经常出现斑点拖尾、色素点分离不清晰等问题.为了避免使用致癌物苯且优化实验效果,选择非极性溶剂石油醚、正己烷、环己烷和极性溶剂乙醇、乙酸乙酯、丙酮多次组合进行薄层色谱分离菠菜色素实验,结果显示:当展开剂为正己烷-丙酮体积比为7:3时分离效果最好,胡萝卜素、脱镁叶绿素、叶绿素a、叶绿素b、叶黄素的Rf值分别为0.92,0.85,0.42,0.28,0.72,色素斑点完整、清晰度好.对该实验方法进行验证,结果显示其重现性和耐用性良好.Thin layer chromatography(TLC)is an important experimental technique for rapid separation and qualitative analysis of a small amount of substances.In the original experiment of separating spinach pigment by TLC,the selected development agent uses a large amount of benzene,a carcinogen,and there usually occur spot tailing and unclear separation of pigment points during the experiment.In order to optimize the experimental effect,avoiding use of carcinogenic benzene,the non-polar solvent petroleum ether,n-hexane,cyclohexane and polar solvent ethanol,ethyl acetate and acetone have been combined for multiple times to separate spinach pigment by thin layer chromatography.The results show that when the volume ratio of n-hexane-acetone is 7:3,the separation effect is the best.When the Rf values of carotene,pheophytin,chlorophyll a,chlorophyll b and lutein are 0.92,0.85,0.42,0.28 and 0.72 respectively,and the pigment spots are complete and clarity.This method has been verified,and the result shows good reproducibility and durability.

关 键 词：薄层色谱法 展开剂 菠菜色素 绿色改进 

分 类 号：O657.7[理学—分析化学]