基于浸泡法的胭脂鱼(Myxocyprinus asiaticus)耳石锶标记技术  

Strontium labeling technique for otolith of Myxocyprinus asiaticus based on immersion method

作　　者：杨祖长 胡望娇 冯广朋[1,2] 张辉 郑跃平[3] YANG Zuchang;HU Wangjiao;FENG Guangpeng;ZHANG Hui;ZHENG Yueping(East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China;Shanghai Yangtze Estuary Fishery Resources Proliferation and Ecological Restoration Engineering Technology Research Center,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China;Shanghai Aquatic Wildlife Conservation and Research Center,Shanghai 202162,China)

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090 [2]上海长江口渔业资源增殖和生态修复工程技术研究中心,上海200090 [3]上海市水生野生动植物保护研究中心,上海202162

出　　处：《中国渔业质量与标准》2024年第2期22-27,共6页Chinese Fishery Quality and Standards

基　　金：国家重点研发计划“蓝色粮仓科技创新”重点专项(2020YFD0900805)。

摘　　要：鱼类规模化标记是水生生物增殖放流的关键技术之一。为探究胭脂鱼(Myxocyprinus asiaticus)增殖放流时耳石锶标记技术的安全性和有效性,以体长(10.47±1.91)cm,体质量为(12.53±5.80)g的胭脂鱼为研究对象,在养殖水体中添加SrCl_(2)·6H_(2)O溶液,使其锶浓度分别达到0、12、18、36和72 mg/L 5个梯度,评估不同锶浓度对胭脂鱼耳石锶标记的效果。结果表明:(1)不同锶浓度下胭脂鱼的死亡率无显著性差异;(2)定量限分析表明,12~72 mg/L锶浓度实验组的Sr/Ca比值在特定区域均出现一个较为明显的峰值,随着外源锶浓度升高,耳石边缘的Sr/Ca值先上升然后趋于平稳;(3)随着标记后时间的变化,肌肉中锶元素含量整体呈现出先升高后降低的趋势,其中12~18 mg/L锶浓度实验组胭脂鱼肌肉中的锶残留可在7 d内完全代谢。综合各项指标表明,耳石锶标记作为胭脂鱼大规模放流的标记技术切实可行,建议选择12 mg/L和7 d作为外源锶的适宜标记浓度和时间。Fish scale labeling is one of the key techniques for the proliferation and release of aquatic organisms.In order to investigate the safety and effectiveness of the strontium otolith labeling technique during the proliferation and release of Myxocyprinus asiaticus,individuals with a mean body length of(10.47±1.91)cm and a mean body weight of(12.53±5.80)g were selected as the study subjects.The strontium concentration in the SrCl_(2)·6H_(2)O solution was progressively increased to five different gradients:0,12,18,36,and 72 mg/L,respectively,to assess the impact of strontium labeling on Myxocyprinus asiaticus at different strontium concentrations.The results showed:(1)No significant differences were observed in the sucker region of Myxocyprinus asiaticus among the different strontium concentrations;(2)Quantitative line analysis revealed that the Sr/Ca ratio in the experimental group exposed to 12-72 mg/L strontium concentration exhibited a distinct peak value in specific areas.As the exogenous strontium concentration increased,the Sr/Ca ratio at the edge of the otolith first increased and then stabilized;(3)Following the change of time after labeling,the content of strontium in muscle showed a trend of initial increasing and then decreasing,with the strontium residue in the muscle of the experimental group,at strontium concentrations of 12-18 mg/L,being completely metabolized within 7 d.It is suggested that a strontium concentration of 12 mg/L and a duration of 7 d should be chosen as optimal conditions for labeling exogenous strontium.

关 键 词：胭脂鱼 耳石 锶标记 锶残留 

分 类 号：S932[农业科学—渔业资源]