瘢痕疙瘩miR-557和eIF2a的表达及靶向关系研究  

Expression and targeting relationship of miR-557 and eIF2a in keloid

作　　者：贾红侠[1] 兰东[2] 于思思[3] 张杰[1] JIA Hongxia;LAN Dong;YU Sisi;ZHANG Jie(Department of Dermatology,Beijing Chao-yang Hospital,Capital Medical University,Beijing 100040,China)

机构地区：[1]首都医科大学附属北京朝阳医院皮肤科,北京100040 [2]首都医科大学附属北京朝阳医院西院皮肤科 [3]首都医科大学附属北京朝阳医院皮肤与医疗美容科

出　　处：《实用皮肤病学杂志》2024年第1期1-7,共7页Journal of Practical Dermatology

摘　　要：目的检测瘢痕疙瘩中微小RNA-557(miR-557)和真核细胞翻译起始因子2a(eIF2a)在病变组织和细胞株中的表达特征,明确两者的靶向关系。方法选择首都医科大学附属北京朝阳医院诊治患者术后的瘢痕疙瘩(n=98)、增生性瘢痕组织(n=49)和正常皮肤组织(n=49)。应用实时荧光定量聚合酶链反应(qRT-PCR)检测miR-557的表达,应用免疫组化检测eIF2a和Ki-67的表达。分离培养瘢痕疙瘩组织中的成纤维细胞,应用双荧光素酶报告基因实验观察miR-57与eIF2a的靶向关系。结果瘢痕疙瘩中miR-557的表达量明显低于增生性瘢痕组织和正常皮肤组织(P<0.05),瘢痕疙瘩中eIF2a表达的阳性率明显高于增生性瘢痕组织和正常皮肤组织(P<0.05),miR-557和eIF2a在不同病变最大径、不同增殖指数和有无伴随症状中的比较差异有统计学意义(P<0.05)。瘢痕疙瘩中miR-557与eIF2a呈负相关性(r=-0.69,P=0.016)。双荧光素酶报告基因实验显示miR-557与eIF2a具有靶向关系。结论miR-557在瘢痕疙瘩中的表达下降,eIF2a的表达升高,均与临床及组织病理特征有关,miR-557与eIF2a具有靶向负调控关系。Objective To detect the expression levels of microRNA-557(miR-557)and eukaryotic translation initiation factor 2a(eIF2a)in keloid tissue and cell lines,and analyze their targeting relationship.Methods Keloid tissues(98 cases),hypertrophic scar tissues(49 cases)and normal skin tissues(49 cases)were collected from patients who underwent treatment at Beijing Chaoyang Hospital.The expression of miR-557 was quantifi ed using real-time quantitative fl uorescence PCR(qRT-PCR),while the expression of eIF2a and Ki-67 was detected through immunohistochemistry(IHC).Fibroblasts derived from keloid tissues were isolated and cultured,followed by the assessment of the targeting relationship between miR-57 and eIF2a by dual luciferase reporter assay.Results The expression of miR-557 was signifi cantly lower in keloid tissues compared to that in hypertrophic scar tissues and normal skin tissues(P<0.05).The expression of eIF2a was signifi cantly higher in keloid tissues compared to that in hypertrophic scar tissues and normal skin tissues(P<0.05).The expression of miR-557 and eIF2a exhibited signifi cant variations across different maximum diameter,proliferation index and accompanied symptoms(P<0.05).Negative correlation was found between miR-557 and eIF2a in keloid tissues(r=-0.69,P=0.016).Targeting relationship was found between miR-557 and eIF2a by dual luciferase reporter assay.Conclusions The expression of miR-557 was downregulated,while the expression of eIF2a was upregulated in keloids,which is associated with clinical and pathological features.Negative targeting regulatory relationship was found between miR-557 and eIF2a in keloids.

关 键 词：瘢痕疙瘩 增生性瘢痕 微小RNA-557 真核翻译起始因子2a 组织病理 靶向关系 

分 类 号：R619.6[医药卫生—外科学]