乙肝肝纤维化患者肝组织黏着斑激酶、蛋白激酶B的表达及意义  

Expression and significance of focal adhesion kinase and protein kinase B in liver tissue of patients with hepatitis B liver fibrosis

作　　者：万亿 孙芳芳 张蔓娜 WAN Yi;SUN Fang-fang;ZHANG Man-na(Department of Infectious Diseases,Guiyang Public Health Treatment Center,Guiyang 550004,Guizhou,China)

机构地区：[1]贵阳市公共卫生救治中心感染科,贵州贵阳550004

出　　处：《川北医学院学报》2024年第5期628-632,共5页Journal of North Sichuan Medical College

基　　金：2019年贵州省卫生健康委科学技术基金项目(gzwjkj2019-1-089)。

摘　　要：目的:探讨乙肝肝纤维化患者肝组织黏着斑激酶(FAK)、蛋白激酶B(AKT)的表达及其意义。方法:纳入75例行肝脏穿刺的慢性乙肝患者作为研究对象,根据纤维化程度(HE和Masson染色)将其分为A组(S0-S1级,n=26)、B组(S2级,n=27)、C组(S3-S4级,n=22)。收集并比较各组患者性别、年龄、总胆红素(TBIL)、肝脏弹性硬度(LSM)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)等一般资料。应用S-P免疫组织化学、Western blot、实时荧光定量PCR检测并比较各组FAK、AKT蛋白及基因水平。结果:各组患者性别、TBIL差异均无统计学意义(P>0.05);各组年龄、LSM、ALT、AST比较,差异均有统计学意义(P<0.05)。免疫组化结果显示,各组患者肝组织中FAK、AKT蛋白阳性表达积分比较:A组<B组<C组(P<0.05),各组p-AKT蛋白表达积分比较:A组>B组>C组(P<0.05)。Western blot结果显示,各组肝组织中FAK、AKT蛋白相对表达水平比较:A组<B组<C组(P<0.05),各组p-FAK、p-AKT蛋白相对表达水平比较:A组>B组>C组(P<0.05)。实时荧光定量PCR结果显示,各组肝组织中FAK、AKT基因mRNA相对表达水平比较:A组<B组<C组(P<0.05)。结论:FAK、AKT表达水平与乙肝患者肝纤维化的发生及肝纤维化程度密切相关。Objective:To investigate the expression and significance of focal adhesion kinase(FAK)and protein kinase B(PKB)in liver tissue of patients with hepatitis B liver fibrosis.Methods:The liver tissues of 75 patients with chronic hepatitis B who underwent liver biopsy were collected and divided into group A(S0-S1 grade,n=26),group B(S2 grade,n=27)and group C(S3-S4 grade,n=22)according to the degree of fibrosis(HE and Masson staining).Baseline data such as gender,age,total bilirubin(TBIL),liver stiffness measurement(LSM),alanine aminotransferase(ALT),and aspartate aminotransferase(AST)were collected and compared among the three groups.The protein and gene levels of FAK and AKT in the three groups were detected by S-P immunohistochemistry,Western blot and real-time fluorescence quantitative PCR,and the differences between the groups were compared.Results:There was no statistically significant difference in gender and TBIL among the three groups(P>0.05).There was a statistically significant difference in age,LSM,ALT,and AST among the three groups(P<0.05).According to S-P immunohistochemistry test,the positive expression scores of FAK and AKT proteins in liver tissues of Group A,Group B,and Group C increased in sequence,while the expression scores of p-AKT protein decreased in sequence(P<0.05).Western blot analysis showed that the relative expression values of FAK and AKT proteins in liver tissues of Group A,Group B,and Group C increased sequentially,while the relative expression values of p-FAK and p-AKT proteins decreased sequentially(P<0.05).After RT-PCF detection,the relative expression values of FAK and AKT gene mRNA in liver tissues of Group A,Group B,and Group C increased sequentially(P<0.05).Conclusion:The expression levels of FAK and AKT are closely related to the occurrence and degree of liver fibrosis in hepatitis B patients.

关 键 词：乙型肝炎 肝纤维化 黏着斑激酶 蛋白激酶B 

分 类 号：R512.62[医药卫生—内科学]