柴杏颗粒的解热止咳作用及其对肺内MPO、NO、iNOS表达的影响  

Antipyretic and Antitussive Effects of Chaixing Granules on Lung the Effect of MPO,NOand iNOS Expression

作　　者：吴迪[1] 王清[1] 张殿文[1] 宋莲莲[1] 常燕 李响 WU Di;WANG Qing;ZHANG Dianwen;SONG Lianian;CHANG Yan;LI Xiang(Jilin Academy of Traditional Chinese Medicine,Changchun 130012,China;College of Basic Medical Science,Jilin University,Changchun 130021,China)

机构地区：[1]吉林省中医药科学院,吉林长春130012 [2]吉林大学基础医学院,吉林长春130021

出　　处：《特产研究》2024年第3期6-11,共6页Special Wild Economic Animal and Plant Research

基　　金：吉林省中医药科技项目(2023054)。

摘　　要：本研究旨在观察柴杏颗粒的解热止咳作用及其对肺内髓过氧化物酶(MPO)、一氧化氮(NO)水平、诱导型一氧化氮合酶(iNOS)蛋白表达的影响。采用小鼠氨水引咳法,取筛选过的ICR小鼠50只,随机分为正常组,小儿柴桂退热颗粒组(5.2g/kg)、柴杏颗粒高、中、低剂量组(3 g/kg、1.5 g/kg、0.75 g/kg),正常组给予蒸馏水,其他组给予相应药液,连续给药3 d,每天1次,末次药后将小鼠放入浓氨水的烧杯内引咳,记录3min内小鼠的咳嗽潜伏期和咳嗽次数;取合格SD大鼠60只,随机分为正常组、模型组、阿司匹林肠溶片组(0.1 g/kg)、柴杏颗粒高、中、低剂量组(2.1 g/kg、1.05 g/kg、0.525 g/kg),正常组和模型组给予蒸馏水,其他组给予相应药液,灌胃给药,每天1次,连续3 d。末次给药后除正常组皮下注射生理盐水外,其他各组皮下注射干酵母混悬液,检测各组温度变化,处死大鼠,测定肺内MPO活性和NO水平,免疫组织化学法检测iNOS蛋白表达。结果表明,与正常组比较,柴杏颗粒各剂量组均有较好的止咳作用(P<0.01),低剂量组对于干酵母致热大鼠具有很好的解热作用(P<0.01或P <0.001);与正常组比较,模型组MPO活性显著增强(P <0.05),NO水平、iNOS表达显著增加(P<0.001),柴杏颗粒高、低剂量组MPO和NO活性下降(P<0.05或P<0.01);柴杏颗粒各剂量组可显著降低肺泡上皮细胞iNOS的表达量(P<0.001)。柴杏颗粒具有良好的止咳作用,其止咳作用机制与降低肺中MPO和NO活性及iNOS的表达有关。To observe the antipyretic and anticough effects of Chaixing granules on the levels of myeloperoxidase(MPO),nitric oxide(NO)and inducable nitric oxide synthase(iNOS)protein expression in lung.50 screened ICR mice were randomly divided into normal group,Xiaoer Chaigui Tuire granule group(5.2 g/kg),Chaixing granule high-dose,medium-dose and low-dose groups(3 g/kg,1.5 g/kg,0.75 g/kg),normal group was given distilled water,and other groups were given corresponding liquid for 3 consecutive days.Once a day,after the last dose,the mice were put into a beaker of concentrated ammonia water to induce cough,and the cough incubation period and cough times of the mice within 3 minutes were recorded.Sixty qualified SD rats were randomly divided into normal group,model group,aspirin enteric co-ated tablet group(0.1 g/kg),Chaixing granule high-dose,medium-dose and low-dose groups(2.1 g/kg,1.05 g/kg,0.525 g/kg).Normal group and model group were given distilled water,and other groups were given corresponding liquid by intragastric administration,once a day.For three consecutive days.After the last administration,the normal group was injected with normal saline subcutaneously,and the other groups were injected with dry yeast suspension subcutaneously.The temperature changes of each group were detected,the rats were killed,MPO activity and NO level in lung were determined,iNOS protein expression was detected by immunohistochemistry.Compared with the normal group,each dose group of Chaixing granule had better antitussive effect(P<0.01),and the low dose group had better antipyretic ef-fect(P<0.01 or P<0.001).Compared with normal group,the MPO activity in model group was significantly increased(P<0.05),the ex-pression of iNOS and NO levels were significantly increased(P<0.001),and the activities of MPO and NO were decreased in high and low dose Chaixing granule groups(P<0.05 or P<0.01).iNOS expression in alveolar epithelial cells was significantly decreased in each dose group of Chaixing granules(P<0.001).Chaixing granules have good an

关 键 词：发热 咳嗽 髓过氧化物酶 一氧化氮 一氧化氮合酶 

分 类 号：R285.5[医药卫生—中药学]