灰楸光合指标的无性系变异及其与环境因子的关系  

Clone Variation of Photosynthetic Index of Catalpa fargesii and Its Relationship with Environmental Factors

作　　者：贠慧玲 杨海裕 张一[2] 麻文俊[3] 李银梅 戴秀芳 YUN Hui-ling;YANG Hai-yu;ZHANG Yi;MA Wen-jun;LI Yin-mei;DAI Xiu-fang(Gansu Xiaolongshan Research Institute of Forest Science,Gansu Xiaolongshan Forest Ecosystem National Research Station,Gansu Province Key Laboratory of Secondary Forest Cultivation,Tianshui 741020,Gansu,China;College of Forestry,Northwest A&F University,Yangling 712100,Shaanxi,China;Research Institute of Forestry,Chinese Academy of Forestry,State Key Laboratory of Forest Tree Genetics and Breeding,Beijing 100091,China)

机构地区：[1]甘肃省小陇山林业科学研究所,甘肃小陇山森林生态系统国家定位观测研究站,甘肃省次生林培育重点实验室,甘肃天水741020 [2]西北农林科技大学林学院,陕西杨陵712100 [3]中国林业科学研究院林业研究所,林木遗传育种国家重点实验室,北京100091

出　　处：《西北林学院学报》2024年第3期138-143,共6页Journal of Northwest Forestry University

基　　金：“十四五”国家重点研发计划(2021YFD2200301)。

摘　　要：分析灰楸无性系光合特性、叶绿素含量与环境因子的关系及遗传变异,旨在鉴定高光合效率、低蒸腾耗散的灰楸优良无性系,为灰楸光合高效育种和优质高效栽培管理提供理论依据。利用LI-6400便携式光合作用测定仪测定光合和气体交换参数,采用SPAD-502叶绿素仪测定叶绿素含量。结果表明,灰楸无性系间净光合速率、蒸腾速率、气孔导度和水分利用效率等4个主要指标存在极显著差异。净光合速率与蒸腾速率、气孔导度、水分利用效率呈极显著正相关,与胞间CO_(2)浓度呈极显著负相关。净光合速率与空气CO_(2)浓度呈负相关。通过回归方程确定了光照强度对瞬时净光合速率、气孔导度、胞间CO_(2)浓度均有较大正贡献。叶绿素含量与瞬时净光合速率呈正显著相关,说明叶绿素含量的高低直接决定楸树光合能力和生长状况。光合指标与环境因子的相关关系显著,光照强度对灰楸无性系瞬时净光合速率影响最大。灰楸无性系光合指标变异系数和重复力均较高,无性系光合能力选择潜力较大。叶绿素含量可作为灰楸早期选育的一个重要参考技术指标,与树高、胸径等共同作为开展灰楸良种选择的关键指标。Photosynthetic characteristics and chlorophyll content are important indicators of physiological characteristics of trees.This study analyzed the relationship between photosynthetic characteristics,chlorophyll content and environmental factors as well as genetic variations of Catalpa fargesii clones,aiming to identify the excellent clones with high photosynthesis efficiency.The parameters of photosynthesis and gas exchange were determined by LI-6400 portable photosynthesis analyzer,and the chlorophyll content was determined by SPAD-502 chlorophyll analyzer.The results indicated that significant differences in net photosynthetic rate(P_(n)),transpiration rate(T_(r)),stomatal conductance(G_(s))and water use efficiency(WUE)existed among the clones.P_(n) was positively correlated with T_(r),G_(s) and WUE,and negatively correlated with intercellular CO_(2) concentration(C_(i)).P_(n) was negatively correlated with the concentration of CO_(2) in the air.According to the regression equation,light intensity has significant and positive contributions to instantaneous P_(n),G_(s) and C_(i).Chlorophyll content was positively correlated with instantaneous P_(n),indicating that chlorophyll content directly determined photosynthetic capacity and growth status of C.fargesii.The correlation between photosynthetic indexes and environmental factors was significant.The variation coefficient and repeatability of photosynthetic indexes of the clones were high,and the selection potential of photosynthetic capacity was large.Together with tree height and DBH,chlorophyll content can be used as a key index for better selection of C.fargesii.

关 键 词：灰楸 光合指标 环境因子 叶绿素含量 遗传变异 

分 类 号：S792.99[农业科学—林木遗传育种]