“88542”水平沟整地措施下土壤微生物群落空间分异特征  

Spatial Differentiation of Soil Microbial Community Under "88542" Horizontal Ditch Preparation Measures

作　　者：胡斯乐 董立国[1] 许浩[1] 韩新生[1] 白晓雄 王月玲[1] 郭永忠[1] 安钰[1] 万海霞[1] 蔡进军[1] HU Si-le;DONG Li-guo;XU Hao;HAN Xin-sheng;BAI Xiao-xiong;WANG Yue-ling;GUO Yong-zhong;AN Yu;WAN Hai-xia;CAI Jin-jun(Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002,Ningxia,China;College of Forestry,Northwest A&F University,Yangling 712100,Shaanxi,China)

机构地区：[1]宁夏农林科学院林业与草地生态研究所,宁夏银川750002 [2]西北农林科技大学林学院,陕西杨陵712100

出　　处：《西北林学院学报》2024年第3期212-220,共9页Journal of Northwest Forestry University

基　　金：宁夏农业高质量发展和生态保护科技创新示范课题(NGSB-2021-14-01、NGSB-2021-14-02);宁夏自然科学基金(2020AAC03293);宁夏重点研发计划(2020BCF01001);宁夏青年拔尖人才培养工程(RQ0025)。

摘　　要：为探明宁夏黄土丘陵区“88542”水平沟整地措施下土壤微生物群落结构的空间分异特征,选取整地措施下形成的水平沟(FL)和上、下相邻水平沟间的隔坡地(GL)表层土壤为对象,通过高通量测序技术,研究整地措施下土壤微生物组成及多样性的变化及其影响环境因素。结果表明,在2种样地共检测到细菌30门、82纲和真菌13门、29纲的微生物。土壤中的主要细菌门为变形菌门、放线菌门和酸杆菌门,主要真菌门为子囊菌门、担子菌门和被孢霉门。2种样地下主要微生物类群的相对丰度存在明显差异,水平沟土壤中变形菌门和担子菌门的相对丰度分别高于隔坡地43.8%和79.6%,隔坡地土壤中放线菌门和子囊菌门的相对丰度分别高于水平沟28.1%和42.1%。α多样性显示,在2种样地间土壤细菌多样性指数无显著差异,但隔坡地上的土壤真菌Shannon、Simpson指数显著高于水平沟。此外,冗余分析显示,脲酶、微生物量碳、有机质以及全N是驱动土壤细菌和真菌群落变化的主要因子。研究结果可为黄土丘陵区整地措施后的生物地球化学过程和微生物变化机制提供理论依据和数据支撑。In order to investigate the spatial differentiation of soil microbial community structure under the"88542"level furrow land preparation measure(a proven successful land preparation method)in the loess hilly area of Ningxia,we selected the surface soil of the level furrow(FL)and the sloping land(GL)between the upper and lower neighbouring level furrows as the research objects,and investigated the changes in the microbial composition and diversity of soil microorganisms and their influences on the environmental factors under the land preparation measures by using high-throughput sequencing technology.The results showed that a total of 30 phyla and 82 classes of bacteria,as well as 13 phyla and 29 classes of fungi,were detected in two sample sites.The dominant bacterial phyla in soil were Proteobacteria,Actinobacteria,and Acidobacteria,while the dominant fungal phyla were Ascomycota,Basidiomycota,and Mucoromycota.There were significant differences in the relative abundance of dominant microbial taxa between the two sample sites.The relative abundances of Proteobacteria and Basidiomycota were higher in the level furrow soil,by 43.8%and 79.6%,respectively,while the relative abundances of Actinobacteria and Ascomycota were higher in the sloping land soil,by 28.1%and 42.1%respectively.Linear discriminant analysis showed that Proteobacteria and Agaricomycetes could serve as indicator microorganisms for the level furrow,while Actinobacteria and Lecanoromycetes could serve as indicator microorganisms for the sloping land.Alpha diversity analysis showed that no significant difference in soil bacterial diversity index between the two sample sites,but the Shannon and Simpson indices of soil fungi were significantly higher in the sloping land.Furthermore,redundancy analysis revealed that urease,microbial carbon,organic matter,and total nitrogen were the main factors driving the changes in soil bacterial and fungal communities.These research results provide a theoretical basis and data support for the biogeochemical processes a

关 键 词：黄土丘陵区 “88542”水平沟整地措施 高通量测序 土壤微生物 

分 类 号：S714.3[农业科学—林学]