功能纳米材料光热抗肿瘤实验设计  

Design of a photothermal antitumor experiment of functional nanomaterials

作　　者：于树玲[1] 许宁宁 刘宇[1] 郑岩 石家华[1] 袁金涛[2] YU Shuling;XU Ningning;LIU Yu;ZHENG Yan;SHI Jiahua;YUAN Jintao(Key Laboratory of Natural Medicine Innovation and Transformation,Henan University,Kaifeng 475004,China;College of Public Health,Zhengzhou University,Zhengzhou 450001,China)

机构地区：[1]河南大学河南省天然药物创新与转化重点实验室,河南开封475004 [2]郑州大学公共卫生学院,河南郑州450001

出　　处：《实验技术与管理》2024年第4期81-88,共8页Experimental Technology and Management

基　　金：2023年河南大学研究生教育教学改革研究与实践项目(YJSJG2023XJ036);2023年度河南省重点研发与推广专项(科技攻关)项目(232102311047);2018年度河南省重点研发与推广专项(科技攻关)项目(182102310589);2023年度河南省高等学校重点科研项目(23B330002)。

摘　　要：将科研课题和功能纳米材料实验教学有机融合,依托校仪器共享平台,设计了光热抗肿瘤综合实验。实验课前以视频、文献和课件等形式介绍背景知识,课堂上进行纳米材料光热抗肿瘤机制讲解,并结合实验目的启发学生自行设计实验方案。实验通过多元评价考核体系,促进学生综合能力提升;将大型仪器使用纳入实验教学,激发学生科研热情;在以科研带教学、以教学促科研方面取得了良好效果。[Objective]The integration of education and scientific research is of great significance in promoting the construction of first-class disciplines and the development of high-level universities.During undergraduate or postgraduate training,combining scientific research and teaching is important in guiding students to discover and explore new knowledge.To improve the quality of student training and build first-class disciplines,gold nanorods(AuNRs)modified with cyclodextrin polyrotaxanes were taken as an example to design a comprehensive experiment to introduce a photothermal antitumor agent.[Methods]Background knowledge of tumors,nanoparticles,and photothermal therapies was introduced via videos,literature studies,and courseware before the experimental class.The mechanism of nanomaterial-based photothermal antitumor therapy was explained in the experimental class.The problem-based learning method was used to consolidate the knowledge of students.Students participating in the experiment were required to prepare AuNRs by the seed growth method from alkyl trimethyl ammonium bromide(CTAB)and sodium oleate.Then,the AuNRs were modified by pseudopolyrotaxane(PPR)with folic acid(FA)terminating at one end and thiolmethoxypoly(ethylene glycol)(mPEG-SH).The morphology and structure of the prepared nanomaterials were characterized by nuclear magnetic resonance spectroscopy(NMR),Fourier transform infrared spectroscopy(FT-IR),ultraviolet-visible spectroscopy(UV-Vis),and transmission electron microscopy(TEM).In vitro photothermal conversion experiments were conducted to evaluate the photothermal conversion properties of the prepared materials.HeLa and HepG2 cells were selected for the photothermal cytotoxicity experiment,and the uptake of AuNR-PPR-PEG-FITC by HeLa and HepG2 cells was observed by inverted fluorescence microscopy(IFM).[Results]The modified gold nanomaterials showed the characteristic absorption peaks of FA at 260,280,and 365 nm,indicating that the AuNRs were successfully modified.Subsequently,they were characteriz

关 键 词：教研融合 光热抗肿瘤 金纳米棒 

分 类 号：R979.1[医药卫生—药品]