辣椒果胶裂解酶基因家族的生物信息学分析  

Bioinformatics Analysis of Pectin Lyase Gene Family in Peppers

作　　者：赵铮 史帅朋 石明军 魏兵强 ZHAO Zheng;SHI Shuaipeng;SHI Mingjun;WEI Bingqiang(College of Horticulture,Gansu Agriculture University,Lanzhou Gansu 730070,China)

机构地区：[1]甘肃农业大学园艺学院,甘肃兰州730070

出　　处：《寒旱农业科学》2024年第5期415-421,共7页Journal of Cold-Arid Agricultural Sciences

基　　金：甘肃农业大学省级大学生创新创业训练计划项目(S202310733050)。

摘　　要：为了解辣椒果胶裂解酶(Pectin lyase like,PLL)基因家族成员数量、理化结构及可能参与的生物学过程,以期为全面鉴定和认识辣椒CaPLLs基因家族的生物学功能提供研究基础。利用全基因组扫描方法和在线生物信息学工具对辣椒CaPLLs基因家族进行了鉴定和分析。结果在辣椒参考基因组的9条染色体上共鉴定出28个CaPLLs基因家族成员,且分别隶属于5个亚族;CaPLLs家族成员的氨基酸序列长度介于183~762 aa,分子量范围为19.65~84.31 kDa,等电点范围为5.39~9.44。保守基序及结构域分析表明,CaPLLs家族成员共有10个保守基序,所有成员均含有Pec_lyase_C结构域。有多种与激素、胁迫应答和生长发育相关的顺式作用元件存在于CaPLLs基因的启动子区域中。由此可见,这28个CaPLLs基因家族成员同一亚家族基因之间高度保守。To understand the number of members of the pectin lyase like(PLL)gene family of pepper,their physicochemical structures,and the biological processes in which they may be involved,the CaPLLs gene family was identified and analyzed using genome-wide scanning and online bioinformatics tools.A total of 28 CaPLLs gene families were identified on nine chromosomes in the reference genome of pepper,belonging to five subfamilies.The length of the amino acid sequence of CaPLLs gene family ranged from 183 to 762 aa,molecular mass ranged from 19.65 to 84.31 kDa,and isoelectric points ranged from 5.39 to 9.44.The analysis of conserved motifs and structural domains showed that there were 10 conserved motifs in CaPLLs family,and all of them contained the Pec_lyase_C domain.A variety of cis-acting elements related to hormones,stress responses,and growth were predicted to be present in the promoter sequences of CaPLLs gene.In this study,28 members of the CaPLLs gene family were identified,and highly conserved among genes in the same subfamily.

关 键 词：辣椒 果胶裂解酶 基因家庭 生物信息学 

分 类 号：S641.3[农业科学—蔬菜学]