实验用大林姬鼠种群微卫星标记开发及遗传特性分析  

Development of microsatellite markers and analysis of genetic characteristics of laboratory Apodemus peninsulae population

作　　者：张晴[1] 张曦文[1] 何松[1] 袁宝[1] 陈健[1] 任文陟[1] 权福实[1] 胡进平[1] 丁瑜[1] ZHANG Qing;ZHANG Xiwen;HE Song;YUAN Bao;CHEN Jian;REN Wenzhi;QUAN Fushi;HU Jinping;DING Yu(College of Animal Science,Jilin University,Changchun 130062,China)

机构地区：[1]吉林大学动物科学学院,长春130062

出　　处：《中国实验动物学报》2024年第4期468-476,共9页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家重点研发计划(2022YFF0710503);吉林省科技发展计划项目(20230505037ZP)。

摘　　要：目的 开发大林姬鼠多态性微卫星标记,丰富大林姬鼠遗传数据,为大林姬鼠遗传质量控制及基因定位等工作奠定基础。方法 基于大林姬鼠基因组序列筛选微卫星位点,挖掘微卫星引物,通过多重PCR技术分析群体的遗传多样性。结果 成功开发出30个微卫星标记,利用60份大林姬鼠基因组DNA对30个微卫星位点进行评价,共检测出152个等位基因,平均每个位点有5.067个等位基因;平均观察杂合度为0.592;平均香农指数为1.265;平均多态信息含量为0.598。结论 基于本研究所开发的微卫星位点具有较好的多态性,能有效分析大林姬鼠群体的遗传多样性,适合为建立大林姬鼠遗传质量标准和遗传质量检测方法奠定基础。Objective Polymorphic microsatellite markers developed for Apodemus peninsulae can enrich its genetic data and lay a foundation for genetic quality control and gene mapping.Methods Microsatellite loci were screened based on the genome sequence of Apodemus peninsulae,and microsatellite primers were identified.The genetic diversity of the population was analyzed by multiplex PCR.Results Thirty microsatellite markers were successfully developed and evaluated using 60 samples of Apodemus peninsulae.A total of 152 alleles were detected,with an average of 5.067 alleles per locus.The average observed heterozygosity was 0.592.The average Shannon index was 1.265.The average polymorphism information content was 0.598.Conclusions Based on the microsatellite loci developed in this study,the genetic diversity of Apodemus peninsulae can be effectively analyzed,laying a foundation for establishing genetic quality standards and detection method.

关 键 词：大林姬鼠 微卫星位点 遗传多样性 封闭群 

分 类 号：Q95-33[生物学—动物学]